绞股蓝内生真菌多样性与其活性成分绞股蓝皂苷A的相关性研究

Diversity of Endogeny Eumycetes in Gynostemma pentaphyllum and Its Correlation with Gypenoside ⅩLⅠⅩ

目的:探讨不同产地不同类型绞股蓝内生真菌多样性与其有效成分绞股蓝皂苷A的关系。方法:采用常规分离法对绞股蓝根茎叶内生真菌进行分离,用插片法和点植法分类鉴定,用HPLC法测定绞股蓝皂苷A含量。结果:绞股蓝根茎叶分离得到的125株内生真菌经过菌落形态、显微特征等鉴定为2纲7目10科22属。镰孢菌属Fusarium是绞股蓝的优势菌属,占总数的22.4%,其次为青霉属Penicillium 12.8%,小球腔菌属Leptosphaeria9.6%。绞股蓝内生真菌种群与绞股蓝皂苷A存在相关性。结论:绞股蓝内生真菌在数量和种群上存在丰富的多样性,而不同内生真菌种群与其品质有关。

Objective：To study the correlation between active components and endogeny eumycetes in Gynostemma pentaphyllum of different types from different habitats. Methods ： Endogeny eumycetes from different parts of C, ynostemma pentaphyllum were isolated by general isolation methods. Insert method and point planting method were used for identification. The content of gypenoside XLIX were determined by HPLC. Results ：125 endogeny eumycetes inhabiting in Gynostemma pentaphyllum were isolated from roots, rhizomes and leaves. By colony morphology and microscopic characteristics ,22 genera from 10 families, 7 orders ,2 classes were identified. Fusarium was the most abundant endogeny eumycetes in Ccnostemma pentaphyllum with the account of 22. 4%. Penicillium and Leptosphaeria was 12. 8% and 9. 6% respectively. The correlation between Gypenoside XLIX and endogeny eumycetes in Gynostemma pentaphyllum was revealed. Conclusion：Endogeny eumycetes are diverse in species and quantity. The endogeny eumycetes species is related to the quality of C, ynostemma pentaphyllum.