布比卡因对高糖培养SH-SY5Y细胞毒性研究

Toxicity study on bupivicaine to SH-SY5Y cells cultured by high-glucose

目的布比卡因作用高糖培养的SH-SY5Y细胞,观测细胞内活性氧(ROS)及凋亡影响。方法 1mmol/L布比卡因作用不同浓度葡萄糖培养基培养的SH-SY5Y细胞6h,检测细胞内ROS、GRP78水平变化及凋亡情况。结果葡萄糖浓度为7.80、11.10、13.30mmol/L组ROS产量均高于5.56、6.10、7.00mmol/L组(P<0.05)。细胞凋亡率组间差异均有统计学意义(P<0.05)。GRP78表达结果为5.56mmol/L组(1.02±0.12)、6.10mmol/L组(0.97±0.06),7.80mmol/L组(0.49±0.04),高于7.00mmol/L组(0.46±0.06);11.10mmol/L组(0.22±0.03)与13.30mmol/L组(0.15±0.07)低于其他各组,差异均有统计学意义(P<0.05)。结论细胞内ROS增多及凋亡增加与布比卡因增强细胞内质网应激(ERS)有关。GRP78功能减弱进一步增强细胞ERS。

Objective To discuss the effect of bupivacaine on SH‐SY5Y cells in high sugar environment and observe the ROS and apoptosis .Methods 1 mmol/L bupivicaine was added to medium with different concentrations of glucose for SH‐SY5Y cells , and the quantity of ROS in the cells and the situation of apoptosis were detected by flow cytometry ,and Western blot was uesd to detect the change of the related protein ,GRP78 .Results The contents of intarcellular ROS in different groups ,which had different concentration（7 .80 ,11 .10 ,13 .30 mmol/L） of sugar medium ,were high than those in groups with concentrations 5 .56 ,6 .10 ,7 .00 mmol/L of sugar medium ,and it showed statistical significance （P〈0 .05） .Cell apoptosis rates among different groups had statisti‐cal significance（P〈0 .05） .Expression of GRP78 in group of 5 .56 ,6 .10 and 7 .80 mmol/L were （1 .02 ± 0 .12） ,（0 .97 ± 0 .06） and （0 .49 ± 0 .04） ,respectively ,and they all were higher than that in group of 7 .00 mmol/L （0 .46 ± 0 .06） .The groups of 11 .10 mmol/L （0 .22 ± 0 .03） and 13 .30 mmol/L （0 .15 ± 0 .07） were lower than the other groups in the expression of GRP78 ,and all the difference shows statistical significance （P〈0 .05） .Conclusion Increasing and apoptosis of intracellular ROS are associated with strengthened endoplasmic reticulum stress by bupivacaine .With the function degrading of GRP78 ,endoplasmic reticulum stress （ERS） may strengthen further .