miR-20b在肾癌中的表达分析及临床意义研究

The expression and clinical significance of miR-20b in renal cell carcinoma

目的检测miR-20b在肾癌及癌旁组织中的表达水平,分析miR-20b的表达与临床病理特征之间的关系,探究其临床意义。方法提取42例手术切除的肾癌及配对癌旁组织标本中的总RNA,经逆转录获得cDNA,通过实时荧光定量PCR检测研究标本中miR-20b的表达量,使用统计学软件分析miR-20b表达的差异及表达水平与患者临床病理特征之间的相关性。结果 miR-20b在肾癌组织中的表达水平明显低于配对癌旁组织(P<0.05),其中有29例(69.048%)肾癌标本miR-20b表达下调。肾癌组织中miR-20b表达量与患者性别、年龄、肾癌组织类型、AJCC临床分期、TNM分期无明显相关(P>0.05)。结论 miR-20b在肾癌组织中的表达明显下调,提示其可能和肾癌的发生发展相关。

Objective To detect the expression level of miR-20b in the renal cell carcinoma （RCC） and paired normal tissues,and analyze the relationships between the expression level of miR-20b and clini-copathologic.Then explore its clinical significance. Methods Total RNA was isolated from 42 RCC tissues and paired adjacent normal tissues resected from nephrectomy ,and cDNA was synthetized by performing reverse transcription.Then real time quantitative PCR（RT-qPCR）was used to detect the expression of miR-20b.We analyzed the relationships between the expression of miR-20b and clinico-pathologic characteristics in tumor and the paired characteristics tissues. Results The expression of miR-20b in RCC tissues was significantly lower than paired adjacent normal tissues（P 〈0.05 ）, and 29（60.048%）RCC tissues showed down-regulation in all tissues which had detectable miR-20b. The results showed that there were no relationships between the expression of miR-20b and patients＇gender,age,histology,TNM stage and AJCC stage. Conclusions miR-20b was down-regulated in RCC tissue,and this indicated that miR-20b might be involved in the tumorigenesis and development of RCC.