参七黄抗结肠癌细胞株SW620作用筛选研究

Study on Constituents from Shenqihuang against Adenocarcinoma of Colon Cell Line SW620 in vitro

目的:对参七黄中的苦参碱等9种组分进行抗结肠癌活性的体外初步筛选。方法:应用MTT法测定各组分对SW620细胞株增殖活性的影响,检测其抑制SW620的剂量效应关系;应用光学显微镜、荧光显微镜进行细胞形态学观察。结果:芦荟大黄素、大黄素、大黄酸、人参皂苷Rh2、槐定碱、苦参碱各浓度均显示出抑制SW620结肠癌细胞增殖的作用,且量效关系明显。结论:此6个单体成分可组成新配伍进行抗癌活性研究,为研发中药治疗结肠癌的靶向新药提供实验依据。

Objective：To study constituents from shenqihuang against adenocarcinoma of colon cell line SW620. Methods： SW620 cell line was treated with Constituents from shenqihuang at various concentrations,The effect on cell Proliferative activity was measured by MTT assay and the effects of alkaloids against cell growth were compared. The changes of cell morphology were observed through optical microscope and fluorescence microscope. Results：Aloe emodin, emodin, rhein, ginsenoside Rh2, Sophoridine matrine were identified to produce growth inhibition against SW620 cells. Conclusion ： The 6 constituents from shenqihuang can form a new compatibility. The results of this study provide experimental evidence for targeting new drug research and development of traditional Chinese medicine treatment for colon cancer.