摘要
目的：采用高效液相色谱（HPLC）法测定不同采收期丹参不同部位丹参酮IIA的含量。方法：采用HPLC法测定，色谱柱为PhenomenexC18柱（4．60mm×250mm，5／μm）；流动相为水（A）-甲醇（B）（75：25）等度洗脱，流速为1mL／min；检测波长为270nm，柱温25℃。结果：丹参酮IIA含量测定线性范围为3．24-97．2μg／mL，R2=0．9999，平均加样回收率为99．1％。结论：不同采收期丹参不同部位丹参酮IIA含量有差异。
Objective： To establish a method for Tanshinone IIA for Salvia miltiorrhiza Bge. Methods：The Phenomenex C18 analytical column （4.60minx 250ram, 5fire） was used. The mobile phases comsisted of H20 and MeOH with isoeratie elution. The flow rate was 1 mL/min,the detection wavelength was at 270 nm and the column temperature was at 25℃. Results： The method had good linear relationship within the range 3.24-97.2μg/mL of Tanshinone IIA, with the correlation coefficent higher as 0. 999 9. The average recovery was 99.1% of Tanshinone IIA. Conclusion： There were certain concentrations of Tanshinone IIA in different parts of Salvia miltiorrhiza Bge in different harvest.
出处
《亚太传统医药》
2015年第12期17-19,共3页
Asia-Pacific Traditional Medicine
关键词
丹参
丹参酮IIA
含量测定
Salvia miltiorrhiza Bge.
Tanshinone IIA
Content Determination
