HPLC法测定乳癖消结丸中丹参酮ⅡA、橙皮苷含量

Content Determination of TanshinoneⅡA and Hesperidin in Rupixiao Node Pills by HPLC method

目的：建立乳癖消结丸中丹参酮ⅡA、橙皮苷高效液相含量测定方法。方法：色谱柱为Diamon—silC18（200mm×4．6mm，5μm），丹参酮ⅡA流动相：甲醇-水（75：25），检测波长：270nm，流速：1mL／min，柱温：30℃；橙皮苷流动相为乙腈-0．2％磷酸溶液（21：79），检测波长：284nm，流速：1mL／min，柱温：25℃。结果：丹参酮ⅡA进样量在0．0403～0．4032ug范围内与峰面积呈良好的线性关系，回归方程为：Y=3E＋06X-38932（r=0．9997），平均回收率为96．4％，RSD为1．6％（n=6）；橙皮苷进样量在0．1157～0．6942Mg范围内与峰面积呈良好的线性关系，回归方程为：Y=787926X=3384．3（r=0．9999），平均回收率为96．9％，RSD为0．9％（n=6）。结论：该方法可用于测定乳癖消结丸中丹参酮ⅡA及橙皮苷的含量，简便稳定、灵敏度高、重现性好，可作为有效控制该药物质量的参考指标。

Objective： To establish the content determination of tanshinone Ⅱ A and hesperidin in Rupixiao node pills by HPLC. Methods：The column was Diamonsil C18 （200mm × 4.6mm, μm）, Tanshinone II A mobile phase was methanol-water （75 ： 25）, detection wavelength was 270nm, flow rate was 1mL/min . The column temperature： 30 ℃. Hesperidin the mobile phase was 0.2 phosphoric acid solution （21 ： 79）, detection wavelength was 284nm, flow rate was lmL/min. The column temperature： 25℃. Resuits ： Tanshinone ⅡA in 0. 040 3μg-0. 403 2μg showed a good linear relationship with the peak area range, The regression equation was： Y=3E＋06X-38932（r=0. 9997）. The average recovery was 96.4%, with RSDof 1.6G（n=6）. Hesperidinin 0.115 7- 0. 694 2fig showed a good linear relationship with the peak area range, The regression equation was： Y-787 926X -3 384.3（r= 0. 999 9）. The average recovery was 96.9 %, with RSD of 0.9% （n= 6）. Conclusion：The method was used to determine the content of nodes in Rupixiao pills of tanshinone A and hesperidin simple and stable, high sensitivity, good reproducibility, Can be used as reference for the effective control of the drug quality.