重组人血管内皮抑制素联合顺铂对人骨肉瘤MG-63细胞VEGF基因表达、增殖及侵袭力的影响

Effects of recombinant human vascular endostatin combined with cisplatin on the expression of VEGF and the proliferation and invasion ability of MG-63 osteosarcoma cells in vitro

目的观察重组人血管内皮抑制素(rh Endo)联合顺铂对人骨肉瘤MG-63细胞VEGF基因表达、增殖及侵袭力的影响。方法顺铂、rh Endo、rh Endo联合顺铂分别处理MG-63细胞,采用RT-PCR和Western blot检测细胞VEGF mRNA和蛋白表达量,CCK-8实验检测细胞增殖,流式细胞术检测细胞凋亡,Transwell小室体外迁移实验检测细胞侵袭和迁移能力。结果 rh Endo联合顺铂组对VEGF mRNA和蛋白表达量、细胞增殖、侵袭迁移的抑制作用以及对细胞凋亡的促进作用均显著高于rh Endo、顺铂单药组,差异有统计学意义(P<0.05)。结论重组人血管内皮抑制素联合顺铂能够在抑制骨肉瘤MG-63细胞VEGF表达、细胞增殖和侵袭力、促进细胞凋亡方面发挥协同作用。

Objective To investigate the effects of recombinant human vascular endostatin（ rh Endo） combined with cisplatin on the expression of vascular endothelial cell growth factor（ VEGF） and proliferation and invasion ability of MG-63 osteosarcoma cells in vitro. Methods MG-63 cells were treated by cisplatin,rh Endo or rh Endo combined with cisplatin,respectively. The expression levels of VEGF mRNA and protein were detected by Western Blot,and the proliferation,apoptosis and invasion ability of osteosarcoma cells were detected by CCK-8 test,flow cytometry and Transwell invasion assay,respectively. Results The expression levels of VEGF mRNA and protein,cell apoptotic rate,the inhibitory effect on cell proliferation and invasion ability in rh Endo combined with cisplatin group were significantly increased,as copmpared with those in simple rh Endo group or simple cisplatin group（ P〈 0. 05）. Conclusion The rh Endo combined with cisplatin can inhibit the expression of VEGF and proliferation and invasion ability of MG-63 osteosarcoma cells,and can promote cell apoptosis,with a synergetic effect.