摘要
目的探讨17β-雌二醇(E2)对人羊膜细胞内β-防御素-4(HBD-4)基因表达的影响。方法体外培养的人羊膜细胞内添加17β-雌二醇(2n M)培养4h、8h、12h、24h,同时各时间点设相应的对照组(Control组),通过real-time RT-PCR技术检测17β-雌二醇对人羊膜细胞内HBD-4 m RNA的表达变化的影响。结果 17β-雌二醇可上调人羊膜细胞中HBD-4 m RNA的表达(P<0.05)。结论 HBD-4在女性妊娠期羊膜细胞宿主免疫防御中起着重要的作用。
Objective We investigated the expression of HBD-4 in human amniotic cells treated with 17β- estradiol （E2）. Methods Expression of HBD-4 in human amniotic cells by stimuli using 17β- estradiol （2nM） for different time 4h, 8h, 12h, 24h was measured by real-time RT-PCR, while a control group in each time point. Results The produnetion of HBD-4 was increased inducibly by 17β-estradiol （P〈0.05）. Conclusion The HBD-4 plays an important role at innate host defense on pregnancy anmiotic cells.
出处
《疾病监测与控制》
2015年第6期380-380,379,共2页
Journal of Diseases Monitor and Control
基金
内蒙古自然科学基金项目(No.2014BS0801)和(No.2012MS1172)
内蒙古医科大学博士启动金
(NY2011BQ003)
内蒙古医科大学青年创业基金(NY2010QN003)
