褐飞虱核糖体蛋白S6激酶基因NlRPS6KA2的克隆及其表达分析

Cloning and expression analysis of a ribosomal protein s6 kinase gene NlRPS6KA2 fromNilaparvata lugens Stl(Hemiptera:Delphacidae)

根据转录组提供的RPS6KA2核心序列信息,应用RACE技术获得了一个编码褐飞虱核糖体蛋白S6激酶的基因NlRPS6KA2的全长cDNA,编码的蛋白含706个氨基酸,具有保守的S_TKc和S_TK_X结构域.荧光定量PCR测定结果表明,NlRPS6KA2基因在褐飞虱若虫和雄虫中表达量均较低,但在怀卵雌虫中大量表达.同时,褐飞虱从感性水稻品种TN1到抗性水稻品种RHT的适应过程中,该基因表达量呈现明显的下降趋势,在适应后有所回升.研究结果为进一步研究NlRPS6KA2基因在褐飞虱中的功能和阐明褐飞虱致害性变异机制提供了依据.

The full-length cDNA ends of NlRPS 6KA 2 gene to code ribosomal protein s6 kinase in Nilaparvata lugens were amplified by RT-PCR according to the RNA-seq analysis of transcriptome; and it encoded a protein of 706 amino acid residues, with two S_TKc and one S _TK_X conservative domains. The real-time quantitative PCR analysis showed that the NlRPS 6KA 2 gene was slightly expressed in nymphs and males, hut was strongly expressed in gravid females. It also showed that the expression level of NlRPS 6KA 2 was down-regulated in the progress of brown planthopper （BPH） adaption from variety TN1 to variety RHT, and the expression level of NlRPS 6KA 2 was slightly up-regulated when BPH was adjusted to RHT. This study was contributes to the further study about this gene＇s function in Nilaparvata lugens. It also puts forward new ideas to explain the virulence changes.