MMP-2、MMP-9及其组织抑制因子在子宫内膜异位症血清及腹腔液中的表达

Expression of MMP-2,MMP-9 and its tissue inhibitors in patients with endometriosis in serum and peritoneal fluid

目的 探讨基质金属蛋白酶-2、-9（MMP-2、MMP-9）及其组织抑制因子-1、-2（TIMP-1、TIMP-2）在不同分期子宫内膜异位症（EMs）患者血清及腹腔液中的表达。方法 收集2011年1月-2013年12月确诊的70例EMs患者和30例对照组血清和腹腔液,用酶联免疫吸附法（ELISA）检测MMP-2、MMP-9、TIMP-1和TIMP-2水平,并进行比较分析。结果 MMP-2和MMP-9在EMs组血清及腹腔液中的表达显著高于对照组、Ⅲ-Ⅳ期显著高于Ⅰ-Ⅱ期、血清中的表达显著高于腹腔液（P〈0.05）。TIMP-1和TIMP-2在对照组血清及腹腔液中的表达显著高于EMs组、Ⅰ-Ⅱ期显著高于Ⅲ-Ⅳ期、腹腔液中的表达显著高于血清（P〈0.05）。结论 MMP-2和MMP-9的高表达、TIMP-1和TIMP-2的低表达在子宫内膜异位症的发病和发展过程中可能起重要的作用。

Objective： To explore the expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in serum and peritoneal fluid of different stage of EMs patients. Methods.. Serum and peritoneal fluid was obtained from 70 cases EMs patients and 30 cases control group from Jan. 2011 to Dec. 2013.Enzyme-linked immunosorbent assay （ELISA） method was used to test the levels of MMP-2, MMP-9, TIMP-1 and TIMP-2, and the results were analyzed in every group. Results： The levels of MMP-2 and MMP-9 in serum and peritoneal fluid in EMs group were significantly higher than that in the control group, they were significantly higher in Ⅲ-Ⅳ stage than that in Ⅰ-Ⅱ stage and in the serum expression were significantly higher than that of peritoneal fluid （P〈0.05） .The levels of TIMP-1 and TIMP-2 in serum and peritoneal fluid in the control group were significantly higher than that in EMs group, they were significantly higher in Ⅰ-Ⅱ stage than that in Ⅲ-Ⅳ stage and in the peritoneal fluid expression were significantly higher than that of serum （P〈0.05） . Conclusion： It may play an important role of MMP-2, MMP-9 high expression and TIMP-1, TIMP-2 low expression in the EMs pathogenesis and development process.