2013～2014年平顶山市流感病毒实验室检测结果分析

Analysis on laboratory test results of influenza virus in Pingdingshan city during 2013-2014

目的分析平顶山市2013~2014年流感病毒实验室检测结果,及时掌握流感病毒类型,为防控工作提供依据。方法采用实时荧光定量反转录聚合酶链反应对哨点医院（平顶山市第一人民医院）采集的流感样病例标本进行核酸检测,并用狗肾传代细胞（MDCK）,通过血凝、血抑实验分离毒株。结果 2013~2014年共检测流感样病例标本1296例,阳性标本231例,总阳性率为17.82%。2013年阳性标本主要集中在1~3月,以新甲型H1N1型流感病毒为主;且11~12月为高发时期,B型流感病毒为绝对优势毒株。2014年阳性标本主要集中在1~3月,以新甲型H1N1型流感病毒为主;且8~12月为高发时期,季节性H3型流感病毒为唯一检出类型。2013~2014年共分离出32株毒株,其中B型Yamagata系7株,新甲型H1N1型1株,季节性H3型24株。结论应及时分析流感病毒特征及流行趋势,尽力避免流感大流行的发生。

Objective To analyze the laboratory test results of influenza virus in Pingdingshan city during 2013-2014 for timely mastering the influenza virus types to provide a basis for its prevention and control work. Methods The throat swabs were collected from the suspected influenza-like cases from the sentinel hospital and performed the nucleic acid detection by the real-time RT-PCR. MDCK was used. The isolated virus was identified by hemagglutination test（HA） and hemagglutination inhibition test（HI）. Results The 1 296 specimens collected from the suspected influenza-like cases were detected in 2013-2014,the positive specimens were 231 cases with the total positive rate of 17.82%. The positive specimens in 2013 mainly concentrated in January to March and was dominated by new influenza A（H1N1） virus,its high epidemic period was from November to December,B type influenza virus was the absolutely predominant strain. The positive specimens during 2014 were mainly concentrated in Jan uary-March,new influenza A（H1N1） virus was predominant strain,moreover the high epidemic period was from August to December and influenza A（H3） virus was the only strain type detected in this period. A total of 32 influenza virus strains were isolated dur-ing 2013-2014,including 7 strain of B Yamagata,1 strain of new A（H1N1） and 24 strains of seasonal H3. Conclusion Should timely analyze the characteristics of influenza virus and epidemic trend and try best to avoid influenza pandemic occurrence.