冬凌草对血浆复钙作用的凝血活性成分

Effect of Coagulant Active Constituents of Rabdosiae Rubescontis Herba on Plasma Recalcification Time

目的:从冬凌草中寻找具有血浆复钙作用的活性成分。方法:对冬凌草乙酸乙酯部位和甲醇部位采用各种柱色谱进行分离纯化,以对体外血浆复钙时间的影响为活性指标,对冬凌草中活性成分进行筛选。结果:从乙酸乙酯部位和甲醇部位共分离鉴定了6个化合物,其中,与维生素K1组相比,冬凌草甲素(1)和迷迭香酸(2)对血浆复钙时间的影响无显著性差异;与空白组相比,咖啡酸(3)对血浆复钙时间的影响无显著性差异;水杨酸(4)(214.77±5.8)s和阿魏酸(5)(233.43±6.88)s对血浆复钙时间小于空白组(252.23±2.6)s,大于维生素K1组(158.2±2.33)s;香草酸(6)(286.2±4.53)s对血浆复钙时间大于空白组,小于灯盏花素组(309.87±2.34)s。结论:冬凌草甲素、迷迭香酸、水杨酸和阿魏酸具有促凝血作用;香草酸具有抗凝血作用。

Objective： To obtain the active compounds of Rabdosiae Rubescontis Herba on plasma recalcification time. Method： Et OAC and methanol extracts were separated and purified by several kinds of chromatography,obserivedthe plasma recalcification time in vitro to screen theactive compounds of Rabdosiae Rubescontis Herba. Result： Six compounds were isolated and identified from the two extracts above. Compared with vitamin K1 group,oridonin（ 1） and rosmarinic acid（ 2） had no significant difference on plasma recalcification time（ P〉0. 05）. Compared with control group（ 200. 87 ± 2. 45） s,caffeic acid（ 3） had no significant difference（ P〉0. 05）. The plasma recalcification times of salicylic acid（ 4）（ 214. 77 ± 5. 8） s andferulic acid（ 5）（ 233. 43 ± 6. 88） s were higherthan that of the control group（ 252. 23 ± 2. 6） s,and lower than that of vitamin K1group（ 158. 2 ± 2. 33） s. The plasma recalcification time of vanillic acid（ 6）（ 286. 2 ± 4. 53） s was longer than that of thecontrol group（ 252. 23 ± 2. 6） s,and lower than that of breviscapine group（ 309. 87 ± 2. 34）s. Conclusion： Oridonin,rosmarinic acid,salicylic acid andferulic acid had procoagulant activity,vanillic acid had anticoagulant effect.