青阳参总苷对慢性不可预知轻度应激大鼠的抗抑郁作用

Effect of Qingyangshenylycosides on Depression in Chronic Unpredictable Mild Stress Rats

目的:本研究评价了青阳参总苷(QYS)在慢性不可预知轻度应激(CUMS)大鼠模型上的抗抑郁活性,并通过研究其对大鼠脑内单胺神经递质浓度的影响探讨QYS的作用机制。方法:将Wistar大鼠随机分为6组,分别为正常组、模型组、氟西汀组和QYS低、中、高剂量组(0.625,2.5,10 mg·kg-1),每组9只。随机给予21 d不可预知刺激建立抑郁症模型,建模同时ig给予相应药物。造模结束后,采用旷场实验观察QYS对大鼠自主活动的影响,采用悬尾实验、强迫游泳实验观察QYS的抗抑郁作用,采用高效液相-电化学检测法测定大鼠脑内单胺递质去甲肾上腺素(NE),5-羟色胺(5-HT)及多巴胺(DA)的浓度。结果:与正常组比较,模型组大鼠悬尾时间及大鼠强迫游泳的不动时间明显延长,自主活动时间明显减少;大鼠脑内额叶皮层中NE,5-HT及DA的浓度明显降低,均具有明显的统计学差异(P<0.05,P<0.01);与模型组比较,QYS(0.625,2.5,10mg·kg-1)能够显著缩短模型大鼠悬尾实验中的不动时间,QYS高剂量组显著缩短模型组大鼠强迫游泳实验中的不动时间,QYS在旷场实验中对模型组大鼠的自主活动无明显影响,QYS高剂量组显著升高模型组大鼠脑内额叶皮层中NE,5-HT及DA的浓度。结论:QYS高剂量对模型抑郁大鼠具有良好的抗抑郁活性,其抗抑郁作用机制可能是QYS提高了模型大鼠脑内额叶皮层NE及5-HT水平。

Objective： To investigate the antidepressant activity of qingyangshenylycosides（ QYS） in the chronic unpredictable mild stress rats（ CUMS）, and to explore its mechanism by the comparison of concentration of monoamine neurotransmitters in brain tissue. Method： A total of 54 male adult Wistar rats were randomly assigned into 6 groups： the normal group,the model group,the fluoxetine group,the low-,medium-and high-dose QYS groups（ 0. 625,2. 5,10 mg·kg- 1） of 9 rats each. The CUMS model was induced in rats by receiving unpredictable mild stress for 21 days. Meanwhile,the corresponding medicines were intragastrically administered to rats. The antidepressant effects of QYS on CUMS rats were evaluated by using the tail suspension test,forced swimming test and open field test. The concentrations of monoamines in rat brain including noradrenaline（ NE）,5-hydroxytryptamine（ 5-HT） and dopamine（ DA） were determined by using highperformance liquid chromatograph with electrochemical detection method. Result： Compared with the normal group,the immobility time in tail suspension and forced swimming test increased, the autonomic activities decreased,the concentrations of NE,5-HT and DA in frontal cortex decreased in the model group. Compared with model group,the immobility time in the tail suspension and forced swimming test decreased,the autonomic activities increased,the concentrations of NE,5-HT increased in the high-dose QYS group（ P〈0. 05,P〈0. 01）.Conclusion： QYS at 10 mg·kg- 1produced reliable antidepressant effect in CUMS rats. The mechanism may be related to improving the concentrations of NE and 5-HT in the CUMS rat brain.