氯化两面针碱对人卵巢癌紫杉醇耐药细胞株A2780 Taxol细胞耐药性的逆转作用

Reversion of Multi-drug Resistance by Nitidine Chloride on A2780 Taxol Cell

目的:研究氯化两面针碱(nitidine chloride,NC)对人卵巢癌紫杉醇耐药细胞株A2780 Taxol细胞耐药性的逆转作用及机制。方法:氯化两面针碱干预A2780 Taxol后,应用MTT比色法检测多柔比星(adriamycin,ADM),依托泊苷(etoposide,VP-16),羟基喜树碱(hydroxycamptothecin,HCPT)对A2780Taxol细胞的抑制率;实时荧光定量PCR检测不同给药组对多药耐药基因(MDR1 mRNA)表达的影响;HE染色法观察肿瘤细胞结构的改变;TUNEL法分析其对细胞凋亡的影响。结果:耐药细胞A2780 Taxol对ADM,VP-16,HCPT的耐药倍数分别为1.87,1.07,3.25;经氯化两面针碱干预后,降低了A2780Taxol细胞对抗肿瘤药ADM,VP-16,HCPT的IC50值,可下降A2780 Taxol细胞的多药耐药(MDR1)基因的表达,HE染色可见NC给药组改变了A2780 Taxol细胞的形态结构,TUNEL染色凋亡细胞明显增多,凋亡指数为(58.03±1.46)%。结论:氯化两面针碱能有效逆转A2780 Taxol多药耐药性,具有良好的抗肿瘤药物多药耐药逆转作用。

Objective： To investigate the reversal or effect of multi-drug resistance by nitidine chloride（ NC） on A2780 Taxol cell. Method： The reversal efficacy of NC was determined by MTT assay. The expressionof multidrug resistance 1（ MDR1） mRNA was assessed by reverse transcription-polymerase chain reaction（ RTPCR）. The structural changes of A2780 Taxol cell were examined with hematoxylin and eosin staining（ HE）.The apoptosis of A2780 Taxol cell was detected by TUNEL assay. Result： The resistance fold of A2780 Taxol cell was 1. 87,1. 07,3. 25 for adriamycin（ ADM）,etopaside（ VP-16）,hydroxycamptothecin（ HCPT）. The 50%inhibition concentration（ IC50） value of A2780 Taxol cell was reduced by NC（ 0. 375,0. 75,1. 5 mg·L- 1）combined with ADM, VP-16 or HCPT,and NC could decrease the expression of MDR1-mRNA,change the structural of A2780 Taxol cell. The apoptosis index（ AI） was（ 58. 03 ± 1. 46） % detected by TUNEL assay.Conclusion： Nitidine chloride can reverse the multidrug resistance of A2780 Taxol cell.