EGCG抑制野百合碱诱导的大鼠右心室肥厚的影响

Inhibiting Effect of EGCG on Right Ventricular Hypertrophy Induced by Monocrotaline in Rats

目的:观察表没食子儿茶素没食子酸酯(EGCG)对野百合碱(MCT)所致大鼠右心室肥厚的影响并探讨其可能的机制。方法:40只雄性SD大鼠随机分为正常组,模型组,EGCG(40 mg·kg-1)组和阳性药卡托普利(25 mg·kg-1)组,每组10只。除正常组外,采用野百合碱诱导制备右心肥厚大鼠模型,24 h后各组分别ig给药,正常组和模型组ig等体积生理盐水,每天1次,连续21 d,观察EGCG对模型大鼠右室肥厚指数(RVHI),右心室质量指数(RVMI),右心室心肌细胞超微结构的变化的影响;对右室心肌组织一氧化氮(NO),诱导性一氧化氮合酶(i NOS),白细胞介素-1β(IL-1β)及IL-6含量的影响。结果:与正常组比较,模型组RVHI,RVMI明显增高(P<0.05),其右心室组织NO,i NOS,IL-1β及IL-6水平明显升高(P<0.05);与模型组比较,EGCG(40 mg·kg-1)组RVHI,RVMI明显降低(P<0.05),右室心肌组织的NO,i NOS,IL-1β及IL-6水平明显降低(P<0.05)。结论:EGCG能有效抑制MCT诱导的大鼠右心室肥厚,其作用途径与其降低右心室心肌组织i NOS表达和NO过多产生及降低IL-1β及IL-6水平有关。

Objective： To observe the effects of epigallocatechin gallate（ EGCG） on right ventricular hypertrophy induced by monocrotaline（ MCT） in rats and explore its mechanisms. Method： Forty male Wistar rats were randomly divided into the normal group,the model group,the captopril group（ 25 mg·kg- 1） and the EGCG（ 40 mg·kg- 1） group. MCT-induced right ventricular hypertrophy model in rats was established,and the effects on right ventricular hypertrophy index（ RVHI）,right ventricular myocardial infarction（ RVMI）,nitric oxide（ NO）,inducible nitric oxide synthase（ i NOS）,interleukin-1β（ IL-1β） and IL-6 of right ventricular hypertrophy myocardium tissue and right ventricular myocardial cells in rats were examined. Result： Compared with the normal group,RVHI and RVMI were significantly elevated（ P〈0. 05）,and NO,i NOS,IL-6 and IL-1βlevel of right ventricular hypertrophy myocardium tissue increased（ P〈0. 05） in the model group. Compared with the model group,RVHI and RVMI was obviously declined（ P〈0. 05）,while levels of NO,i NOS,IL-6 and IL-1βsignificantly decreased（ P〈0. 05） in the EGCG group. Conclusion： EGCG could significantly inhibit the RVH induced by MCT in rats. Its mechanisms may be related to reducing the levels of i NOS,NO,IL-1β and IL-6 in right ventricular hypertrophy myocardium tissue.