体内和体外条件下不同浓度硅酸钠和对应pH抑制粉红单端孢(Trichothecium roseum)效果比较

Comparison of inhibitory effect of sodium silicate at different concentrations and corresponding p H on Trichothecium roseum in vitro and in vivo

本文研究了不同浓度硅酸钠和对应p H对T.roseum孢子萌发和菌落生长的抑制;100mmol/L硅酸钠和对应p H12.60处理孢子3,6,9h后,损伤接种苹果对其病斑直径的影响;以及采用扫描电镜观察处理后孢子表面形态的变化。结果表明,不同浓度硅酸钠和对应p H处理均能有效抑制T.roseum孢子的萌发和菌落生长,但硅酸钠处理的效果要更好(p<0.05),硅酸钠处理后的孢子其病斑直径明显低于对照(p<0.05),但经对应p H12.60处理后的孢子其病斑直径则与对照相比无显著性差异(p>0.05)。硅酸钠处理的孢子表面粗糙,皱缩明显,表面结构破坏,有明显的内容物渗出;对应p H处理虽然也能导致孢子皱缩,但并未破坏其结构。上述结果表明,硅酸钠比对应p H处理对T.roseum生长的抑制作用更为明显。

The inhibitory effect were assayed on spore germination and colony growth of T.roseum treated with sodium silicate at different concentration and corresponding pH.The lesion diameter was determined in apple fruits inoculated with pores treated with 100mmol/L sodium silicate and corresponding pH12.60 for 3, 6 and 9h. Morphology of spores treated with 100mmol/L sodium silicate and corresponding pHI2.60 was observed by SEM. The results showed that sodium silicate at different concentration and corresponding pH significantly inhibited the spore germination and colony growth. However, sodium silicate treatments showed more effective than corresponding pH treatments（p 〈0.05）.Apples inoculated with spores treated with sodium silicate had a smaller lesion diameter when compared with the control;however, no difference was found in lesion diameter treated with corresponding pH and the control. Spores treated with sodium silicate appeared rough, significant shrinkage, damaged structure and contents exudate. The treatment with corresponding pH also caused the shrinkage of spores, but the structure was not damaged. It was suggested that sodium silicate treatment caused more severe damage than corresponding pH treatment against T.roseum.