转MvNHX1和MvP5CS基因棉花耐盐抗旱性比较与育种价值分析

Comparative Analysis on Drought and Salt Resistance and Breeding Value in Transgenic MvNHX1 and MvP5CS Cotton

通过对花粉管通道法获得的T7转MvNHX1基因的10个棉花株系和转MvP5CS基因的3个棉花株系与对照非转基因棉花D5在温室内盐和干旱胁迫下的发芽率、生理生化指标以及田间花期干旱胁迫下农艺性状和纤维品质进行分析发现:温室内盐和干旱胁迫后,转基因棉花叶片叶绿素含量、脯氨酸含量均高于对照,而丙二醛含量低于对照;田间花期干旱胁迫下,2种转基因植株果枝数、有效果枝数、铃数、有效铃数、铃重、子棉、皮棉、衣分、子指和衣指均高于对照,说明转MvNHX1基因和转MvP5CS基因植株在干旱逆境下的产量高于非转基因;经花期干旱胁迫后,2种转基因棉花的纤维断裂伸长率、短纤维率、马克隆值和纺纱一致性指数优于非转基因棉花D5。综合分析表明:2种转基因棉花的耐盐抗旱性均有提高,其中转MvNHX1基因棉花的耐盐性优于转MvP5CS基因棉花植株,而转MvP5CS基因棉花植株的抗旱性优于转MvNHX1基因棉花植株。

We studied 10 transgenic lines of MvNHX1 and three transgenic lines of MvP5 CS, all of which were the T7 genera- tions obtained from the way of pollen tube channel. The non-transgenic cotton D5 was used as a control, and the study took place in a greenhouse and in the field. Under conditions of drought and salt stress in the greenhouse, we measured the germina- tion rate, chlorophyll content, proline content and MDA content of the MvNH.X1 transgenic lines, the MvP5 CS transgenic lines and the D5 control. We found that the chlorophyll and proline content of transgenic lines were higher than the control and the MDA content was lower. Under conditions of drought stress in the florescence in the field, we found that branches, the effective branches, bolls, the effective bolls, the boll weight and the lint of the two transgenic lines exceeded the control. Consequently, the productivity of transgenic cotton was found to be higher during drought stress. Furthermore, elongation at break, short fiber content, micronaire and spinning consistency index of the two transgenic lines were higher than in the D5 control. The results of the two transgenic lines showed that the MvNHX1 lines had advantages in terms of salt tolerance and that the MvP5CS lines were superior in drought tolerance.