摘要
目的构建一种新型的磁靶向定位灌注化疗膀胱癌的吡柔比星纳米制剂,并探讨其磁靶向定位及抗膀胱癌细胞特性,为定位灌注化疗膀胱癌的临床应用提供帮助。方法通过N-N'-羰基二咪唑(CDI)交联剂将治疗膀胱癌的临床药物吡柔比星(pirarubicin,THP)连接在表面氨基化的四氧化三铁(Fe3O4)磁性纳米粒上,制备具有磁性的THP纳米制剂,并在体外考察其磁定位性能和对膀胱癌细胞的抑制作用。结果 THP成功地连接到了粒径40 nm左右的Fe3O4磁性纳米粒上,磁性药物制剂在不同p H值的缓冲液中药物在90%以上,并可在外界磁场作用下定位于靶向部位。对膀胱癌细胞可造成THP和Fe3O4双重抑制效果,抑制率高达58.44%。结论该纳米制剂可通过外界磁场定位于靶向部位,膀胱癌细胞抑制效果明显,为临床定位灌注化疗的应用奠定了基础。
Objective We synthesized novel magnetic pirarubicin nanoparticles for targeted intravesical chemotherapy to treat bladder cancer .Methods Pirarubicin was grafted on Fe 3 O4 nanoparticles which were surface-functionalized with amino-groups via N, N′-Carbonyldiimidazoleas targeted intravesical infusion chemotherapy agents.Mimetic position in vitro by external magnetic field and inhibition to bladder cancer cells of prepared agents were demonstrated.Results Pirarubicin was successfully grafted on magnetic Fe3 O4 nanoparticles whose size were around 40 nm.The residual of pirarubicin on the pharmaceutic agents was above 90% in buffer solutions of various pH.They also could be positioned to certain area by external magnetic. Meanwhile, the prepared magnetic pirarubicin nanoparticles gave multiple-inhibition to bladder cancer cells by THP and Fe3 O4.The inhibition rate was up to 58.44% after co-cultured for 2 h.Conclusions These magnetic pirarubicin nanoparticles have a great potential for clinical use in infusion chemotherapy.
出处
《北京生物医学工程》
2015年第3期267-272,共6页
Beijing Biomedical Engineering
基金
重庆市万州区科委科技计划项目(201303005)
重庆大学大学生科研训练计划项目资助
关键词
膀胱癌
吡柔比星
FE3O4
灌注化疗
磁靶向
bladder cancer
pirarubicin
infusion chemotherapy
magnetic targeting