甘蓝型油菜青杂7号实验室快速纯度鉴定技术体系的建立

Construction of seed purity inspection technology system of rapeseed hybrid( Brassica napus L. ) Qingza No. 7

为了建立准确高效的青杂7号杂交种种子纯度鉴定方法,并促进青杂7号的推广。本研究选用青杂7号的恢复系、不育系及F1杂交种,利用60对SSR引物对其进行分析并利用大田制种群体对标记进行验证。同时对碱裂解DNA快速提取方法进行了优化。结果表明:得到的1对SSR共显性标记P49,该标记的鉴定结果与大田鉴定结果基本一致,但SSR标记检测方法比大田检测更快更准确;优化的DNA快速提取方法提取的DNA可以稳定扩增出清晰可见的条带,可以应用于鉴定油菜杂交种纯度,进一步加快了油菜杂交种纯度鉴定的速度。

In order to establish the technology for seed purity inspection of Qingza No .7 and promote the extension of Qingza No .7.The fertile lines, sterile lines and F1 of Qingza No.7 were selected and 60 pairs of simple sequence repeat （ SSR） primers were used for analyzing .The populations of field seed producing were used to verify .At the same time , the alkaline lysis method is optimized . One co-dominant marker P49 was identified and the result was consistent with field identification . The co-dominant marker P49 was more efficient to distinguish false hybrids compared with field ob-servation method. Fragments can be steadily amplified from the DNA extracted by the modified alka-line lysis method .It is confirmed that the modified alkaline lysis method is suitable to be applied in the large-scale hybrid seed purity inspection of rapeseed , which would speed up hybrid seed purity inspection of rapeseed with SSR marker .