双向薄层色谱法分析混合糖组分

Two-step thin layer chromatography for saccharides analysis of mixed saccharides liquid

薄层色谱法分析糖类混合物的组分简便快速,但是并不能将甘露糖、阿拉伯糖、半乳糖、木糖、葡萄糖进行彻底分离.本文采用双向层析的方法分别以V乙腈∶V水=85∶15为第一向展层剂,以V正丁醇∶V乙酸乙酯∶V水=7∶2∶1为第二向展层剂优化层析.结果显示,得到了较好的分离效果,可以定性检测出糖类组分.对比国内外研究,此方法简单,因此双向薄层色谱法可以作为一种简单、准确、清晰、便利的方法定性检测糖类混合物的组分.

The thin layer chromatography（ TLC） analysis of the mixed saccharides liquid composition effect is good,simple and rapid,but it doesn＇ t put the mannose,arabinose,galactose,xylose,glucose separately. This paper using the two step- TLC,the first step by Vacetonitrile∶ Vwater= 85 ∶ 15,and the second step by Vn- butanol∶ Vethyl acetate∶VWater= 7∶ 2∶ 1 as developing solvent for optimization of chromatography. Results show that using the above method to analyze saccharides mixture, got the good separation effect, can the qualitative detection of saccharides components,comparative research both at home and abroad,the use effect of the method is very simple and intuitive. Therefore,the two step thin layer chromatography（ TLC） can be used as a simple,accurate,clear and convenient method of qualitative detection of saccharide mixture components.