草莓果实中七次跨膜受体蛋白生物信息学及时空表达特征分析

Bioinformatics and expressional analysis of 7 transmembrane receptor proteins in strawberry(Sweet Charlie)fruits

以草莓为试材,基于拟南芥(Arabidopsis thaliana)、人(Homo sapiens)和家鼠(Mus musculus)等数据库信息,采用同源比对方法,结合ClustalX 2.0序列比对及MEGA 5.0系统进化树分析,对草莓基因组中7TMR的基础生物信息进行了分析。结果表明,草莓基因组中含有84个7TMR,包括23个亚家族。半定量RT-PCR分析表明,该家族全部成员中有33个成员随果实发育表达量明显上调,17个成员表达量明显下调;Real-time PCR分析进一步确认,部分7TMR成员的基因表达与果实发育及成熟过程的整个进程有着密切的关系。此外还发现,草莓中7TMR家族中含有一类特殊的亚家族蛋白,该亚家族与人和家鼠中的脂联素受体高度同源,表明草莓中7TMR存在着非G-蛋白偶联信号转导途径。草莓中类似脂联素受体亚家族ADIPOR包含5个成员,对这5个成员的时空表达和刺激应答进行了分析,发现其中某些成员不仅与果实发育进程关系密切,且对糖信号应答,暗示着该类蛋白在草莓果实发育和成熟调控中可能起着重要作用。

Based on the homology search of the strawberry database with that of Arabidopsis thaliana, Homo sapiens and Mus musculus, the 7TMR protein family in strawberry plants （Sweet Chartie） was characterized using CtustatX 2.0 and MEGA 5.0 phylogenetic tree analysis software. The results showed that the 7TMR protein family in strawberry contained 84 members that constituted 23 sub-families. Semi-quantitative PCR analysis indicated that, among the 84 members,the transcript levels of 33 members significantly increased,whereas 17 members significantly decreased in their transcript levels along with fruit growth and development. The expression patterns of 6 up-regulated and 4 down- regulated genes were further demonstrated by using real-time PCR. It was important for that among the 7TMR protein family, a special subfamily was highly identical to that of adiponectin receptors. This adiponectin receptors-like subfamily （ADIPOR） constituted of 5 members. Real-time PCR analysis indicated that the expression of some adiponectin receptors-like members was correlated to fruit growth and development, and the response to sucrose and glucose treatment as well, implying their important roles in sugar signaling during strawberry fruit growth and development. This study was of great significance in further revealing the molecular mechanisms as well as the function of 7TMR proteins in strawberry fruit growth and development.