摘要
为探究侧脑室注射Nesfatin-1对雌性大鼠促性腺激素水平及其mRNA表达的影响,对初情期前雌性大鼠的侧脑室注射Nesfatin-1,采用实时荧光定量PCR法检测促黄体素(Luteinizing hormone,LH)和促卵泡素(Folliclestimulating hormone,FSH)mRNA在垂体中的变化,采用酶联免疫分析法检测血清LH和FSH的浓度。结果表明:在初情期前的雌性大鼠中,Nesfatin-1可促进初情期启动、增加卵巢重量、血清LH水平、垂体LH mRNA和FSH mRNA的表达增加(P<0.05);但Nesfatin-1对FSH水平的影响不突出,注射后15min时增加(P<0.05),60min不产生影响(P>0.05)。侧脑室注射Nesfatin-1后15和60min均能提高血清LH水平,15min时提高更明显(P<0.05),但对FSH水平的影响不尽相同。在成年的雌性大鼠中,Nesfatin-1对LH和FSH的分泌不产生影响(P>0.05)。Nesfatin-1可通过下丘脑诱导和增强初情期过渡阶段雌性大鼠LH和FSH的释放及LH、FSH mRNA的表达。
To study the effect of nesfatin-1 on gonadotropin secretion and their mRNA expression by intracerebroventricular injection at the central level in rats. Female rats at pre-puberty were treated with nesfatin-1 by intracerebroventricular injection. The luteinizing hormone (LH) and follicle-stimulating hormone (FSH) mRNA expression in pituitary were determined with real-time quantitative PCR. The concentration of serum LH and FSH were determined with enzyme-linked immunosorbent (ELISA). The results showed that, nesfatin-1 could significantly advance vaginal opening,enhance ovarian weight,serum LH level and mRNA expression of LH, FSH in pituitary during the pre-puberty (P〈0.05) ;but FSH level was significantly increased at 15 minutes after intracerebroventricular injection (P〈0.05) but not at 60 minutes after intracerebroventricular injection (P〉0.05). Serum LH level was increased at 15 and 60 minutes after intracerebroventricular injection of nesfatin-1 ,where LH level was increased more at 15 min compared to 60 min (P〈0.05). Nesfatin-1 could not affect the secretion of gonadotropin in adult rats (P〉0.05). Nesfatin-1 could induce and enhance the secretion and mRNA expression of FSH and LH through the hypothalamus during the pubertal transition.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2015年第3期139-145,共7页
Journal of China Agricultural University
基金
国家自然科学基金资助项目(31472096)
高等学校博士学土点专项科研基金博导类资助课题(20123418110004)
关键词
大鼠
NESFATIN-1
促黄体素
促卵泡素
侧脑室注射
rat
nesfatin-1
luteinizing hormone (LH)
folricle-stimulating hormone (FSH)
intracerebroventricularinjection