G250mAb修饰的肿瘤细胞靶向基因载体功能研究

Study on vector function of G250 mAb modified tumor cell target gene

目的分析G250m Ab与聚乙烯亚胺(PEI)偶联作为肿瘤靶向基因载体系统的优越性,为肿瘤细胞靶向基因治疗提供研究基础。方法通过二硫键将G250m Ab与PEI偶联,得到修饰后的基因载体G250m Ab-PEI,利用体外转染细胞的方法研究其转基因的优越性。结果 G250m Ab-PEI转染效率明显高于单纯PEI或传统脂质体转染;其毒性也较未修饰的PEI低;对人宫颈癌细胞Hela的基因转染具有显著的靶向性,其转基因效率是肝癌细胞Hep G2(G250阴性)的2倍,而对正常血管平滑肌细胞的基因转染效率较Hela低近20倍。结论 G250m Ab-PEI是一种高效、低毒和具有靶向性的基因载体。

Objective To research and analyze the superiority of G250mAb coupling with polyethyleneimine （PEI） as a tumor-targeted gene vector to lay the foundation for targeted gene therapy of tumor cells. Methods G250mAb was coupled with PEI by the disulfide bond for obtaining the modified gene vector G250mAb-PEI. The transfected cells in vitro method was applied to study its transgenetic superiority. Results The transfection efficiency of G250mAb-PEI was significantly higher than that of pure PEI or traditional lipofectamine trasfection;its toxicity was lower than that of non-modified PEI;the gene transfection of cer-vical cancer Hela cells had significant targeting and its transfection efficiency was twice that of liver cancer cells HepG2 （G250 negative）,but the transfection efficiency of normal vascular smooth muscle cells （SMC） was lower than that of Hela by nearly 20-fold. Conclusion G250mAb-PEI is a highly efficient gene vector with low cytotoxicity and targeting effect.