金纳米粒子-石墨烯修饰玻碳电极的制备及对多巴胺、抗坏血酸和尿酸的同时检测

Preparation of Gold Nanoparticles-Graphene Modified Glassy Carbon Electrode and Its Application for Simultaneous Determination of Dopamine,Ascorbic Acid and Uric Acid

采用三步法制备了金纳米粒子-石墨烯层层组装的复合材料,并将其修饰在玻碳电极上,制备成一种新型的同时检测抗坏血酸（AA）、多巴胺（DA）和尿酸（UA）的电化学传感器。采用扫描电子显微镜（SEM）对复合材料进行了表征,并研究了传感器对AA、DA、UA电催化性能。结果表明：该传感器对AA、DA和UA的氧化具有很好的催化和分离效果,可实现AA、DA和UA的同时测定。在三者共存体系中,AA-DA、DA-UA、AA-UA的氧化峰电位差分别为152mV、161mV和313mV。线性范围分别为1.996×10^-5~5.580×10^-3、1.996×10^-6~5.478×10^-3和1.000×10^-6~1.000×10^-3 mol/L,检出限分别为1.200×10^-5、1.030×10^-7和4.100×10^-7 mol/L。该修饰电极选择性好、稳定性高,有望用于实际样品中AA、DA和UA的同时检测。

Gold nanoparticles/graphene composites were prepared by three-step method and modified on the glassy carbon electrode,then a new kind of sensor for the simultaneous detection of ascorbic acid（AA）,dopamine（DA）,uric acid（UA）was fabricated.The composite materials were characterized by scanning electron microscope（SEM）.And the catalytic performance of AA,DA and UA at the modified electrode was studied.The results showed that the sensor exhibited good catalytic effect on the oxidation of AA,DA and UA with separated oxidative potentials.The composite film modified electrode can be used for the simultaneous determination of AA,DA and UA.In the coexisting system of AA,DA and UA,the differences of the oxidation peak potential of AA and DA,DA and UA,AA and UA were 152,161 and 313mV,respectively.For the simultaneous detection of AA,DA and UA,the linear ranges were1.996×10^-5-5.580×10^-3,1.996×10^-6-5.478×10^-3 and 1.000×10^-6-1.000×10^-3 mol/L,with the detection limit of 1.200×10^-5,1.030×10^-7 and 4.100×10^-7 mol/L,respectively.The modified electrode has good selectivity,high stability,and is expected to be used for the detection of AA,DA and UA in the real samples.