摘要
研究了槟榔多糖粗提物(ASP)的抗氧化活性及其对人皮肤成纤维细胞(HSF)内氧化损伤的抑制作用。结果表明:利用超声波辅助法提取的槟榔子多糖经初步去杂质后,具有良好的1,1-二苯基-2-三硝基苯肼(DPPH·)清除能力、二价铁螯合能力和三价铁还原力。在对HSF细胞内氧化损伤的抑制作用研究中,当过氧化氢(H2O2)处理浓度为150μmol/L、ASP添加浓度为20μg/m L时,HSF细胞存活率上升至93.15%,与对照组差异不显著(p<0.05);当短波紫外线照射强度为100μW/cm2、照射时间为3.5 h、ASP添加量为20μg/m L时,HSF细胞存活率上升至82.92%,与对照组差异不显著(p<0.05)。因此,ASP具有抑制H2O2和短波紫外线处理对HSF细胞造成的氧化损伤的作用。
The in vitro antioxidant activities and protective effects on human skin fibroblasts （HSF） of crude polysaccharides from Areca catechu L. seed were evaluated. The initially purified ASP had good DPPH radical scavenging acitivity, ferrous ions chelating capacity and strong reducing power. Cell culture results indicated that HSF（damaged by H202 or UV C） treated with ASP exhibited better cellular morphology and higher cell variability compared with the negative control group, and the protective effects were stronger with a supplemented ASP dose in the experimental range.
出处
《热带作物学报》
CSCD
北大核心
2015年第6期1136-1141,共6页
Chinese Journal of Tropical Crops
基金
中央级公益性科研院所基本科研业务费专项(No.1630052015040)
海南省自然科学基金项目(No.314146)
关键词
槟榔多糖
抗氧化
抗细胞内氧化损伤
Polysaccharides from Areca catechu L. seed
Antioxidant activities
Protective effects
