拟南芥核膜孔蛋白Nup160基因的突变体表型分析

Phenotype Analysis of Mutants of Nucleoporin 160 Gene in Arabidopsis

植物开花受环境条件的影响,其分子调控机制在转录、翻译和表观遗传等层次已有较广泛而深入的研究;核膜孔复合物是真核细胞中细胞质与细胞核之间物质交换的重要通道。筛选到拟南芥核膜孔蛋白Nup(Nucleoporin)160基因的3个突变体nup160-1/-2/-3,均表现出发育的多种表型,最显著的表型为早花,而且是不依赖于环境条件(光周期和温度)的早花。但是,突变体在长日下的开花时间依然早于短日条件下的开花时间,暗示突变体仍然对光周期有反应。结果表明,在拟南芥中Nup160作为一个植物开花的负调控因子,主要在温敏途径和光周期途径中参与拟南芥开花时间的调节。

Plant flowering is affected by environmental conditions, and its molecular regulatory mechnism in transcription, translation and epigenetics levels has been widely studied. Nuclear pore complex （NPC） is a key access of substance communication between cell nucleus and cytoplasm. This experiment screened nup160-1/-2/-3 mutants of nucleoporin 160 （Nupl60）, showing a variety of development phenotypes. The most significant one was early flowering regardless of environmental conditions （photoperiod and temperature）. However, the nupl60 mutants flower earlier in long day conditions than in short day conditions, indicating that nup160 mutants still had response to photoperiod. The results suggested that Nupl60, as a negative regulator, played a role both in the thermosensory pathway and photoperiod pathway to manipulate flowering time of Arabidopsis.