激光熔融静电纺丝法制备PLLA及PLLA/nHA纤维支架及性能研究

Preparation of PLLA and PLLA/nHA scaffolds by laser melt electrospinning and biomedical application

利用激光熔融静电纺丝方法制备了PLLA和PLLA/nHA纤维支架,采用FTIR和DSC测试对支架材料的结构和热学性能进行表征,通过熔融电纺对PLLA和PLLA/nHA纤维支架进行体外降解实验,研究了失重率与降解时间的关系。同时对激光熔融和一般溶液电纺得到的PLLA和PLLA/nHA纤维支架进行细胞相容性实验,对两种方法所得支架的安全性进行评价。结果表明:nHA对PLLA的结构和晶型产生影响,并减缓PLLA的降解速度,激光熔融电纺支架更具安全性,其更适合组织工程应用。

Poly-L-lactide（PLLA）and Poly-L-lactide/nano-hydroxyapatite（PLLA/nHA）scaffolds were prepared via laser melt electrospinning technique.The structure and thermal properties of electrospun fibers were investigated by FTIR and DSC.The weight loss were observed during the degradation in 7weeks.Human embryo kidney cells（HEK293cells）were seeded on the electrospun and melt electrospun scaffolds respectively,and the cell growth situation was evaluated by inverted fluorescence microscope and MTT test.The result indicated that the structure and crystal form of PLLA were changed due to the addition of nHA.The laser melt electrospinning as a promising method to fabricate scaffolds was much safer compared with the conventional solution electrospinning.The nHA particles can improve the cell-compatibility of composite scaffolds in both electrospinning and melt electrospinning.