导入opaque-2基因的微胚乳玉米氨基酸含量的研究

Studies on the Amino Acids Content of the Micro-endosperm Maize with opaque-2 Gene

以6个导入opaque-2(o2)基因的微胚乳玉米(简称o2微胚乳玉米)为材料,自交4代分别获得自交系S1、S2、S3、S4,随机抽样S1和S4的部分籽粒,测定其各种氨基酸含量。结果表明,o2微胚乳玉米各品系经4代自交后,氨基酸含量发生了增减两个方向的变化。1号、2号品系自交4代后,氨基酸总量、必需氨基酸总量都略有降低或保持不变,而3号、4号品系自交4代后氨基酸总量、必需氨基酸总量都有较大提升。o2微胚乳玉米自交4代后,除品系1外,各品系S4代都比S1代赖氨酸含量提高,并且差异达显著水平。S1与S4间氨基酸总量相关系数、必需氨基酸总量相关系数及赖氨酸含量相关系数均为正值,但相关关系都不显著。

6 micro-endosperm maize materials with opaque-2 （o 2） gene （o 2 micro-endosperm maize for short） were used in this study,which have been inbred 4 generations to obtain S1 ,S2 ,S3 and S4.A part of grains were selected from S1 and S4 in random sampling,amino acids were measured.The main results were as follows：after the inbreeding was conducted for 4 generations in the o 2 micro-endo- sperm maize,their amino acid （AA） content was changed.When they were inbred 4 generations,their AA content and essential amino acids （EAA） content in strain 1 and strain 2 were slightly lower or unchanged,but the AA content and EAA content in strain 3 and strain 4 were increased.After the o 2 micro-endosperm maize was inbred 4 generations,the lysine content of each strain in the S4 was higher than that in the S1 except strain 1 ,and the t was significant at 0.05 level.All of the simple correlation coefficients of AA,EAA and lysine content between S1 and S4 were positive but not significant at 0.05 level.