FC/TK对大鼠卵巢癌的免疫治疗作用的实验研究

Experimental study on immune therapeutic effects of FC / TK on rat ovarian cancer

目的:探讨自杀性肿瘤疫苗(FC/TK)对大鼠卵巢癌的治疗作用。方法:采用聚乙二醇法,将自然杀伤基因修饰的卵巢癌细胞与树突状细胞融合制备成FC/TK,选取40只雌性Fischer344大鼠,制备Fischer344大鼠卵巢癌荷瘤模型,采用随机数字表法分为FC/TK组(FC/TK处理)、未经自然杀伤基因修饰的卵巢癌细胞与树突状细胞融合的疫苗(FC)处理组(FC组)、经60Co照射的FC/TK处理组(60Co-FC/TK组)和对照组(等量PBS处理)各10只。计算各组大鼠90天后的肿瘤平均重量、抑瘤率等指标。结果:FC组、FC/TK组的瘤体重量(3.17±0.68)g、(2.92±0.59)g均显著低于60Co-FC/TK组和PBS组且差异均具有统计学意义(P<0.05);FC组、FC/TK组的抑瘤率(61.53±10.28)%、(64.56±11.77)%显著高于60Co-FC/TK组且差异具有统计学意义(P<0.05)。FC组、FC/TK组的血清IL-2、TNF-ɑ水平显著低于60Co-FC/TK组和PBS组且差异均具有统计学意义(P<0.05)。FC组、FC/TK组的瘤体实质中CD4+、CD4+/CD8+比例显著的高于60Co-FC/TK组和PBS组,CD8+细胞显著的低于60Co-FC/TK组和PBS组且差异均具有统计学意义(P<0.05)。结论:FC/TK对大鼠卵巢癌具有显著的治疗作用,可能和提高肿瘤实质内部的T淋巴细胞亚群水平、降低血清炎症因子水平有关。

Objective： To investigate the Dutch act tumor vaccine （FC/TK） treatment effect on rat ovarian cancer. Methods： U- sing poly （ethylene glycol）, natural killer gene modified ovarian cancer cells with dendritic cells were prepared by the fusion of a FC/TK. 40 female Fischer 344 rats were selected to prepare Fischer344 rats ovarian cancer tumor model and were randomly divided into FC/TK group （FC/TK processing）, the suicide gene modified ovarian cancer cells with dendritic cell fusion vaccine （FC） processing group （FC group）, the FC/TK 60Co irradiation treatment group （60Co-FC/TK group） and control group （the same amount of PBS treatment）, 10 rats in each. After 90 days, the average tumor weight, inhibition rate and other indicators were calculated. Results： Tumor weight of FC group and FC/TK group was （3. 17±0. 68） g, （292± 0. 59 ） g, which were significantly lower than that of 60Co-FC/TK group and PBS group and the difference was statistically significant （P〈0. 05）. The tumor inhibition rate of FC group and FC/TK group was （61.53±10. 28）%, （64. 56± 11.77）% , which was higher than that of 60Co-FC/TK group and the difference was significant. Serum IL-2 and TNF alpha levels of the FC group and FC/TK group were significantly lower than those of 60Co-FC/TK group and PBS group and the differences were statisti- cally significant （P〈0. 05） . CD4 ＋ and ratio of CD4 ＋ / CD8 ＋ in tumor essence of FC group and FC/TK group were higher than those of 60Co-FC/TK group and PBS group, CD8 ＋ cells of FC group and FC/TK group were significantly lower than those of 60Co-FC/TK group and PBS group and the difference was statistically significant （P〈O. 05 ） . Conclusion： FC/TK on rat ovarian cancer has significant thera- peutic effect and may improve tumor parenchyma internal T lymphocyte subsets level, reduce the serum levels of inflammatory cytokines. [