昆明芦荟根腐病病原菌的分离及鉴定

Isolation and Identification of Aloe Root Rot Pathogens in Kunming

芦荟具有较高的经济和药用价值,研究芦荟根腐病的病原菌有利于芦荟产业的发展。采用纯培养技术对芦荟根腐病的病原菌进行分离鉴定,并利用离体根部接种法对其进行致病性测定。结果表明,从感染芦荟的根中分离得到一株根腐病病原菌;依据其形态结构特征和ITS测序初步鉴定为尖孢镰刀菌。致病性测定发现,利用分离出病原菌接种的芦荟根段均有病斑,其中刺伤和无刺伤的根段的病斑率分别高达94.4%和66.7%。

Aloe chinensis has high economic and medicinal value, but the occurance of root rot greatly limits the development of the Aloe chinensis industry. Using pure culture techniques, the Aloe chinensis root rot pathogen was isolated and identified, and its pathogenicity was further assessed using an in vitro root inoculation method. One root rot pathogen fungus was isolated from the infected roots of Aloe chinensis; based on ITS morphological structure characteristics and ITS gene, the fungal isolate was preliminary identified as Fusarium oxysporum. Pathogenic de- termination showed that the inoculated strains of Aloe chinensis root segment has diseased spots. Diseased spot rati- os were found to be 94. 4 % and 66.7 % using the stab and no-stab root methods, respectively.