小半夏汤对化疗性异食癖模型大鼠血清胃肠激素的影响

Effect of Xiaobanxia Decoction on Gastrointestinal Hormone in Chemotherapy-induced Pica in Model Rats

目的:观察小半夏汤对化疗大鼠胃肠激素水平的影响,以探讨其防治化疗性恶心呕吐的机制。方法:雄性Wistar大鼠60只,随机分为空白对照组、小半夏汤正常对照组、顺铂模型组、昂丹司琼组、小半夏汤大剂量组和小剂量组。除前两组腹腔注射等容量生理盐水外,其余各组腹腔注射顺铂6 mg/kg建立化疗性异食癖模型。造模前1 h及造模后11 h分别灌胃给药,每次各组均以10 m L/kg容积给以蒸馏水、小半夏汤0.8 g/kg、蒸馏水、昂丹司琼1.3 mg/kg、小半夏汤1.6 g/kg、小半夏汤0.8 g/kg。每12 h记录动物高岭土摄入量。造模24 h后取血,酶联免疫法检测血清胃肠激素水平。结果:模型组动物摄食高岭土量显著升高,外周血中胃泌素和胃动素水平降低、生长抑素与血管活性肠肽升高。小半夏汤在减少模型动物摄食高岭土的同时,可升高胃泌素和胃动素水平(P<0.05)、降低生长抑素与血管活性肠肽水平(P<0.05)。结论:小半夏汤防治化疗性恶心呕吐作用机制与其改善胃肠激素紊乱有关。

Objective：In order to explore the anti- emetic mechanism of Xiaobanxia decoction（XBXT）,its effect on gastrointestinal hormone level was observed in chemotherapy- induced pica rats. Methods：Sixty male Wistar rats were randomly divided into normal control,XBXT control,cisplatin model,ondansetron,XBXT high dose and low dose groups. Pica model was induced by cisplatin 6 mg/kg i.p.. One hour before and 11 hour after cisplatin injection,each group was gavaged with distilled water,XBXT 0.8 g/kg,ondansetron 1.3 mg/kg,distilled water,XBXT 1.6 g/kg and 0.8 g/kg respectively. After 24 h of cisplatin injection,gastrointestinal hormones in serum were measured using ELISA method. Results：Both dosages of XBXT could significantly inhibit rat pica（P0.01）,and reverse the abnormal increase of somatostatin and VIP（P〈0.05） and abnormal decrease of gastrin and motilin（P〈0.05）. Conclusion：The mechanism of XBXT on the treatment of chemotherapy- induced nausea and vomiting was related to ameliorate the disorders of gastrointestinal hormone.