DBP基因rs7041位点多态性与汉族人群肥胖易感性的关系

Association of DBP gene rs7041 locus polymorphism with susceptibility to obesity in Han population

目的：探讨维生素D结合蛋白（DBP）基因rs7041位点多态性与汉族人群肥胖易感性的关系。方法：采用PCR·RFLP技术检测211例肥胖者和213例体重正常者的DBP基因rs7041位点基因型，采用logistic回归分析基因多态性与肥胖的关联性，采用多因素降维法分析基因一环境之间的交互作用。结果：肥胖组和对照组DBP基因rs7041位点TG＋GG和TT基因型分布频率差异有统计学意义（X2=5．425，P=0．020），肥胖组rs7041位点等位基因G分布频率高于对照组（x2=5．175，P=0．023）。调整相关影响因素后，DBP基因rs7041位点TG基因型携带者肥胖危险性升高（调整OR=1．528，95％CI=1．017—2．295），TG＋GG基因型携带者肥胖危险性升高（调整OR=1．605，95％CI=1．079～2．389）。进一步分析显示，DBP基因与环境因素（吸烟、饮酒、体力活动等）之间无交互作用（P〉0．05）。结论：DBP基因rs7041位点多态性与肥胖易感性之间存在关联。

Aim： To explore the association of vitamin D binding protein（ DBP） gene rs7041 locus polymorphism with the susceptibility of obesity in Han population. Methods： The single nucleotide polymorphism of DBP gene rs7041 in 211 obese individuals and 213 controls were detected using PCR-RFLP. The association between DBP gene polymorphism and obesity was estimated by logistic regression,and interaction of gene-environment was evaluated using the non-parametric multifactor dimensionality reduction model. Results： Significant difference was found in the gene type TG ＋ GG and TT frequency of rs7041 between obesity and control groups（ χ2= 5. 425,P = 0. 020）. The frequency of G allele in obesity group was higher than that of control group（ χ2= 5. 175,P = 0. 023）. Further analysis revealed that TG and TG ＋ GG of DBP gene rs7041 polymorphism increased the risk of obesity after adjusting age,gender,etc（ adjusted OR = 1. 528,95%CI = 1. 017- 2. 295; adjusted OR = 1. 605,95% CI = 1. 079- 2. 389）. Gene-environment interaction analysis presented no interaction between DBP gene and environmental factors（ smoking,alcohol consumption,physical activity,etc）（ P〈0. 05）. Conclusion： DBP gene rs7041 polymorphism is associated with the risk of obesity.