抗菌性壳聚糖双胍醋酸盐的辐照辅助制备及其生物毒性

Irradiation Assisted Preparation of Antimicrobial Chitosan Biguanidine Acetate and Its Biological Toxicity

在γ-射线预辐照的壳聚糖(CTS)上引入双胍基合成了具有抗菌性能的壳聚糖双胍醋酸盐(CBA)。研究了吸收剂量与合成条件对CBA产率的影响,用傅里叶变换红外光谱(FT-IR)和核磁共振碳谱(13C-NMR)表征CTS和CBA的结构。测定了CBA对金黄色葡萄球菌及白色念珠菌的最小抑菌浓度,测试了CBA的小鼠经口摄入毒性和兔皮肤敷涂刺激性。结果表明:辐照能有效提高CBA的产率,且不改变CBA的化学结构。CBA具有与壳聚糖双胍盐酸盐(CBH)相当的抗菌性,且无摄入毒性和皮肤刺激性。

Chitosan biguanidine acetate （CBA） was synthesized by introducing biguanidine onto γ-ray preirradiated chitosan （CTS）. The effect of absorbed dose and synthesis condition on the yield of CBA were investigated. The structures of CTS and CBA were characterized by Fourier Transform Infrared spectroscopy （FT-IR） and carbon-13 Nuclear Magnetic Resonance spectrum （^13C-NMR）. Antimicrobial property of CBA against staphylococcus aureus and monilia albican were tested . Toxicity for oral intake of mice and coating irritation on rabbit skin of CBA were evaluated. Results indicate that the yield of CBA increases evidently after irradiation of CTS. However, the chemical structure of CBA has no change. The antimicrobial property of CBA is equal to chitosan biguanidine hydrochloride（CBH）, and CBA is non-toxic and nonirritant for skin.