生物大分子印迹聚合膜的表征及电化学应用

Biological Macromolecular Characterization and Electrochemical Applications of Molecularly Imprinted Polymer Film

使用扫描电子显微镜（SEM）、红外图谱（IR）、循环伏安（CV）、差示脉冲循环伏安（DPV）对血红蛋白聚合物膜修饰电极进行了表征。 DPV测试表明血红蛋白的浓度和峰电流分别在低浓度和高浓度下均有良好的线性关系，低浓度下满足线性回归方程ip（μA）＝13.05－3.58C（μM），相关系数R为－0.9960。检出限为2.7×10^－9 M，线性范围8.0×10^－9～7.1×10^－7 M；高浓度下满足线性回归方程Ip（μA）＝10.56－0.1001C（μM），相关系数R为－0.9957。检出限为5.7×10^－7 M，线性范围1.7×10^－6～1.4×10^－5 M。可用于构建生物分子印迹的电化学传感器。

The structure and properties of hemoglobin modified electrode （ BHb/MIP-CPE ） were characterized by scanning electron microscopy （ SEM ） , IR spectrum , cyclic voltammograms （ CV ） , differential pulse voltammograms （ DPV）.The sensor was tested by differential pulse voltammetry （ DPV ） to investigate the relationship between the response current and concentration.The results showed that a wide detection linear range of 8.0 ×10^-9 ~7.1 ×10^-7 M and 1.7 ×10^-6 ~1.4 ×10^-5 M for the determination of hemoglobin in low concentration and high concentration with the low detection limit of 2.7 ×10^-9 M （ S/N=3 ） and 5.7 ×10^-7 M （ S/N=3 ） were obtained , respectively.Paper successfully constructed electrochemical sensor based on biological molecular imprinting.