大鼠肌肉挫伤后COX6C mRNA的表达与损伤时间关系

Relation between Injury Time and the Expression of COX6C mRNA in Skeletal Muscle of Rats after Contusion

摘要：目的探讨肌肉挫伤后组织中细胞色素c氧化酶亚基VIc（cytochromecoxidasesubunitVIc，COX6C）mRNA的表达变化及其与损伤时间的关系。方法54只健康成年SD大鼠随机被分为正常对照组和损伤后0．5、1、6、12、18、24、30和36h组，采用重力锤自由落体方式制作大鼠肌肉挫伤模型。于相应时间提取挫伤处肌肉组织样本进行常规组织学观察，并提取组织中总RNA采用real-time PCR检测COX6C mRNA相对表达量。结果损伤后6h内仅见肌纤维间出血、肌细胞肿胀等改变，未见炎症细胞浸润。损伤6h以后逐渐出现肌细胞变性、坏死、炎症细胞浸润、纤维结缔组织增生等改变。COX6C mRNA在损伤后6h以前其表达量均高于正常对照组，而在损伤6～36h，其表达量均低于正常对照组。结论COX6C mRNA在大鼠肌肉挫伤后呈现规律性表达．结合组织学检查有助于损伤时间的推断和研究。

Objective To investigate the relation between injury time and the expression of cytochrome c oxidase subunit Vie （COX6C） mRNA in skeletal muscle of rat after contusion. Methods A total of fifty- four SD rats were divided into the control group and the contusion groups （0.5, 1, 6, 12, 18, 24, 30, and 36h after contusion）, randomly. The contusion model was established by free fall drop of gravity ham- mer. At corresponding time point after contusion, the regular histology was examined and expression level of COX6C mRNA was tested by real-time PCR after extraction of total RNA from the tissues. Results The main pathological features of 6 h after injury included edema and hemorrhage in myocytes with no inflammatory cells found. After 6 hours, the findings included myocyte degeneration and necro- sis, inflammatory cells infiltration, and fibrous connective tissue proliferation in the contused zone. The expression level of COX6C mRNA was higher than that of the control group within 6 h after contusion. The expression level was lower than that of the control group from 6-36 h after contusion. Conclusion The level of COX6C mRNA expresses in a regular way after contusion. It may be useful for estimating wound age in combination with the results of laathological features.