尿液中γ-羟基丁酸及其前体物质的检测和应用

Analysis of GHB and Its Precursors in Urine and Their Forensic Application

目的建立尿液中γ-羟基丁酸（gamma-hydroxybutyric acid，GHB）及其前体物质1，4-丁二醇（1，4-butanediol，1，4-BD）和γ-丁内酯（gamma-butyrolactone，GBL）的液相色谱一串联质谱法（LC-MS／MS），为相关案件提供依据。方法以GHB-d6、MOR-（13为内标，尿样经甲醇沉淀蛋白后通过液相色谱分离，电喷雾离子源进行离子化，多反应监测模式对各化合物进行检测。结果GHB及其前体物质1,4-BD、GBL的检出限分别为0．1、0．1和2~g／mL，准确度为87．6％-98．1％，日内及日间精密度均小于15％，基质效应大于80％。结论所建立的分析方法灵敏度高、简便快速、专属性强、可靠性高，可为司法鉴定实践中涉及GHB的案件提供技术支持和基础数据。

Objective To establish the method to analyze γ-hydroxybutyric acid （GHB） and its precursors 1,4-butanediol （1,4-BD） and gamma-butyrolactone （GBL） in urine through LC-MS/MS and provide evi- dence for related cases. Methods GHB-d＋ and MOR-d3 were used as the internal standard. The urine sample was separated by LC after protein precipitation with methanol. The electrospray ion source was for ionization. Each compound was detected through multiple-reaction monitoring （MRM） mode. Results The limits of detection of GHB and its precursors 1,4-BD and GBL were 0.1, 0.1 and 2 μg/mL. The accuracy was 87.6%-98.1%. The intra-day and inter-day precisions were less than 15% and matrix ef- fects were higher than 80%. Conclusion The method is high sensitive, simple, rapid, specific and with high reliability. This study has provided technical support and basic data for forensic cases involving GHB.