桑叶多糖调节小鼠肠道菌群失调的研究

Regulation of Morus alba polysaccharides on disturbance of intestinal flora in mice

目的研究桑叶多糖对小鼠肠道菌群失调的调节作用。方法利用盐酸林可霉素诱导小鼠肠道菌群失调。将造模成功的小鼠随机分为桑叶多糖组、乳酶生组、模型组,另设置对照组。桑叶多糖组小鼠ig 6.0 mg/m L桑叶多糖溶液0.6 m L(剂量0.144 mg/g),乳酶生组小鼠ig 5.5 mg/m L乳酶生混悬液0.6 m L(剂量0.132 mg/g)。2次/d,连续给药7 d。模型组、对照组小鼠自行饮水。取小鼠的新鲜粪便,提取肠道菌DNA。16S段DNA序列扩增,变性梯度凝胶电泳(DGGE)法分析。比对各条带及样品,进行聚类分析,对各组小鼠肠道微生物群落结构进行主成分分析。结果 DGGE直观图分析,经过自然恢复、桑叶多糖和乳酶生的作用,小鼠肠道菌群种类有了明显的增加,但与对照组小鼠的肠道菌群比较仍然有差别,并不能完全恢复到原来的水平。聚类分析结果可以看出,与对照组最接近的是乳酶生组,其次是桑叶多糖组,最后是自然恢复组。菌群落结构的主成分分析可以直观地看出,桑叶多糖、乳酶生组小鼠肠道菌群与对照组关系最近,模型组与对照组肠道菌群及其他组肠道菌群的差异明显。结论桑叶多糖对小鼠肠道菌群失调有调节作用。

Objective To study the regulation ofMorus alba polysaccharides on disturbance of intestinal flora in mice.Methods Models of disturbance of intestinal flora in mice were induced by lincomycin hydrochloride. Mice with disturbance of intestinal flora were randomly divided intoM. alba polysaccharides group, lactasin group, and model group. And other rats were selected as the control group. The mice in the M. alba polysaccharides group were ig administered with 6.0 mg/mLM. alba polysaccharides 0.6 mL （equivalent to dosage 0.144 mg/g）, and those in lactasin group were ig administered with 5.5 mg/mL Lactasin Tablets 0.6 mL （equivalent to dosage 0.132 mg/g）. The animals were given drugs twice daily, and lasted for 7 d. Mice in model and control groups were fed with water at the same time. DNA of intestinal flora was extracted from the fresh excrement of the mice. 16S DNA sequence amplification was done, and the analysis was determined via denaturing gradient gel electrophoresis （DGGE） method. Strips of samples were observed, the clustering analysis was carried out, and principal component analysis （PCA） method was used to analyze structures of intestinal flora in mice.Results It could be observed from DGGE that the species of intestinal flora in mice obviously increased after natural recovery, or treated byM. alba polysaccharides and lactasin. But there was still difference of the number of intestinal flora in mice between these three groups and the control group. The intestinal flora could not completely resume to the original level. The cluster analysis of these samples showed that control group was close to lactasin group, secondlyM. alba polysaccharides, and then natural recovery group. Flora community structure PCA results of control group were near to those ofM. alba polysaccharides and lactasin groups, however there were significant differences between model group and control group and other group.ConclusionM. alba polysaccharides has regulatory effects on disturbance of intestinal flora in mice.