减压贮藏对杏鲍菇采后活性氧代谢的影响

Effects of Hypobaric Storage on Reactive Oxygen Species Metabolism of Pleurotus eryngii During Cold Storage

为探讨减压贮藏调控杏鲍菇采后衰老的作用机理,本文研究了30、70 k Pa和常压3个贮藏压力对冷藏杏鲍菇的活性氧代谢影响。试验结果表明:30 k Pa的减压贮藏能显著减缓杏鲍菇体硬度的下降,维持较高的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)等抗氧化酶活性,抑制超氧阴离子(O2·-)的生成速率和过氧化氢(H2O2)含量增加,减少丙二醛(MDA)的积累和细胞膜透性的升高,延缓菇体衰老。适宜的减压处理可以诱导抗氧化酶活性的提高,有效维持杏鲍菇采后活性氧代谢平衡,抑制膜脂过氧化作用,从而延缓了菇体采后衰老进程,延长贮藏期。研究结果为减压贮藏在果蔬贮藏上的应用提供理论依据。

To investigate the effects of different hypobaric conditions（ 30 k Pa,70 k Pa） on reactive oxygen species metabolism of Pleurotus eryngii,we determined the change of the firmness,activities of SOD,CAT,POD and APX,O2·^-production rate,contents of MDA and H2O2,and relative electric conductivity. The results showed that the Pleurotus eryngii that treated by hypobaric storage（ 30 k Pa） could maintaina high level of firmness,increased activities of SOD,CAT,POD and APX significantly,and inhibited the increase of O2·^-production rate,H2O2,relative electric conductivity and MDA content. Because induced high antioxidant enzymes activities,which was beneficial to remove the excess accumulation of free radicals and protect the cell membrane system integrity,hypobaric storage（ 30 k Pa） treatment could postpone the senescence of Pleurotus eryngii. This s The results of the study provide theoretica basis for hypobaric storage applying to fruits and vegetables.