Reprimo 和 hMLH1基因甲基化在胃癌早期诊断价值的研究

Research on Reprimo,hMLH1 Gene Methylation in Early Diagnosis Value of Gastric Cancer

目的：探讨 Reprimo 和 hMLH1基因启动子区甲基化检测在胃癌早期诊断中的价值。方法通过陕西省人民医院2013年9月-2014年4月收治的慢性萎缩性胃炎伴肠上皮化生患者50例、异型增生患者50例、胃癌患者50例，取内镜下胃活检组织，并取正常胃黏膜活检组织30例作为对照组，分析 Reprimo 基因和 hMLH1基因启动子区甲基化表达情况，比较各组患者间的差异。结果患者组的胃黏膜组织中 Reprimo 基因和 hMLH1基因启动子区甲基化阳性率显著高于正常组，差异有统计学意义（P ＜0.05）。Reprimo 基因启动子区甲基化阳性率：慢性萎缩性胃炎伴肠上皮化生组28％（χ^2＝10.18，P ＜0.05）；异型增生组56％（χ^2＝25.84，P ＜0.05）；胃癌组62％（χ^2＝30.36，P ＜0.05）；hMLH1基因启动子区甲基化阳性率：慢性萎缩性胃炎伴肠上皮化生组20％（χ^2＝4.39，P ＜0.05）；异型增生组44％（χ^2＝15.13，P ＜0.05）；胃癌组48％（χ^2＝17.41，P ＜0.05），检测的特异度高。结论Reprimo 和 hMLH1基因甲基化检测在胃癌早期诊断中的价值很高，特异度高，是一种有效的诊断方式，在患者的临床诊断方面拥有广阔的治疗前景。

Objective To investigate the Reprimo and hMLH1 gene promoter methylation detection value in the diagnosis of early gastric cancer.Methods Chose patints in Shaanxi Provincial People’s Hospital from September 2013 to April 2014,50 cases of patients with chronic atrophicgastritis with intestinal metaplasia,50 cases of patients with gastricmucosal atypical hyperplasia,50 patients with gastric cancer,endoscopic gastric biopsy samples,and 30 cases of normal gastric mucosa biopsy tissues as control group.Analysis abnormal expression in Reprimo gene and hMLH1 genes promoter methylation,compared the differences.between groups of patients.Results The patients with gastric mucosatissues Reprimo and hMLH1 genes promoter methylation positive rate was significantly higher than that of normal group,the difference wasstatistically signifi-cant （P〈0.05）.Reprimo gene promoter methylation were：patients with chronic atrophic gastritis with intestinal metaplasia 28% （χ^2 =10.18,P 〈 0.05）.Patients with gastric mucosalatypical hyperplasia 56% （χ^2 = 25.84,P 〈 0.05）and patients with gastric cancer 62% （χ^2 = 30.36,P 〈 0.05）.hMLH1 gene promoter methylation were：patients with chronic atrophic gastritis with intestinal metaplasia 20% （χ^2 =4.39,P 〈0.05）,patients with gastric mucosal atypical hyperplasia 44% （χ^2 =15.13,P 〈0.05）and patients with gastric cancer 48% （χ^2 = 17.41,P 〈0.05）,high specificity of detection.Conclusion Reprimo and hMLH1 gene’s detect value in the diagnosis of early gastric cancer is very high,high specificity,it is an effec-tive way of diagnosis,treatment in clinical diagnosis of patients with broad prospects.