临床患者三种性传播疾病分子生物学检测分析

Clinical Molecular Biology Analysis of Three Kinds of Sexually Transmitted Diseases in Patients

目的：分析就诊于北京协和医院患者沙眼衣原体（chlamydia trachomatis，CT）、淋病奈瑟菌（neisseria gonorrhoeae， NG）、解脲脲原体（ureaplasma urealyticum，UU）三种性传播疾病的检出情况以及相关因素。方法收集2013年1月1日-2014年12月31日两年间样本，采用实时荧光核酸恒温扩增检测技术（simultaneous amplification and testing，SAT）进行CT-RNA，NG-RNA 和 UU-RNA 的检测并分析。结果6262例送检样本中，2013年2412例，2014年3850例。CT-RNA，NG-RNA 和 UU-RNA 的阳性率：2013年分别为：7.29％，3.44％和38.21％；2014年分别为8.17％，4.15％和45.71％，三种病原体阳性率，2014年均高于2013年，其中 UU-RNA 的阳性率在两年间的差异具有统计学意义（χ^2＝12.66，P ＜0.01）。两年间的 CT-RNA，NG-RNA，UU-RNA 的总阳性率分别为8.15％，3.89％，42.77％；UU-RNA 在女性当中的阳性率为61.05％，远高于男性的阳性率24.50％，其差异具有统计学意义（χ^2＝316.13，P ＜0.01）。CT-RNA，NG-RNA，UU-RNA 阳性患者最多见于21-30岁；UU-RNA 在宫颈拭子样本中检出率最高。结论在这三种性传播疾病中UU 感染率增高的趋势最为明显；CT 和 NG 较易感染男性，UU 则易感染女性；CT，NG，UU 病原体在青年中的感染率最高。

Objective To analysis the related factors and the morbidity of patients with Chlamydia trachomatis（CT）,Neisse-ria gonorrhea（NG）,Ureaplasma urealyticum （UU）in Beijing Union Medical College Hospital.Methods Simultaneous Am-plification and Testing （SAT）was used to detect CT-RNA,NG-RNA,UU-RNA of patients from January of 2013 to Decem-ber of 2014.Results There were 6 262 cases in all and 2 412 cases in 2013,and 3 850 cases in 2014.The positive rate of CT-RNA,NG-RNA and UU-RNA were 7.29%,3.44% and 38.21% in 2013.The positive rate of CT-RNA,NG-RNA and UU-RNA were 8.17%,4.15% and 45.71% in 2014 respectively,which were higher than those of in 2013.There was a signifi-cant difference in UU-RNA positive rate between the two years （χ^2 =12.66,P 〈0.01）.The positive rate of CT-RNA,NG-RNA and UU-RNA were 8.15%,3.89% and 42.77% respectively in two years.The positive rate of UU-RNA in women’s （61.05%）was significantly higher than the men’s （24.50%）（χ^2 =316.13,P 〈0.01）.Most of the people with CT-RNA, NG-RNA and UU-RNA-positive were between 21 to 30 years old.The highest detection positive rate was in cervical swabs samples of UU-RNA.Conclusion There was a significantly increasing trend of the infection with UU.Male were more sus-ceptible to CT and NG.However,female were more susceptible to UU.There was the highest infection rate in youth （21-30 years）with CT,NG and UU.