摘要
为了探讨不同接种途径和日龄对SPF鸡感染J亚群禽白血病病毒(ALV-J)后感染模式的影响,本研究分别经11日龄鸡胚尿囊腔、1日龄口服、点眼滴鼻、腿部肌肉注射、颈部皮下注射、腹腔注射和7日龄腹腔注射途径接种了ALV-J CHN06株,于不同时间点(第1、2、4、6周),分别对各试验组的病毒血症、抗体及排毒进行了动态检测。结果显示,11日龄鸡胚尿囊腔组100%(15/15)感染模式为V+S+。1日龄口服组和点眼滴鼻组感染模式以V-S-为主。1日龄颈部皮下注射组、腿部肌肉注射组和腹腔注射组在第4、6周的感染模式均以V+S+为主。7日龄腹腔注射组在试验期内仅呈现一过性病毒血症,而且7日龄感染组在第6周的抗体阳性率比1日龄感染组高,差异显著(P<0.05)。本研究结果表明,胚胎感染易导致持续性病毒血症,注射途径比非注射途径更易诱导病毒血症和排毒。随着感染日龄的增加,病毒血症持续时间缩短,排毒时间推后,抗体生成水平升高;防止ALV-J早期感染有利于ALV-J的防控和净化。
To understand the effects of inoculation route and age on infection pattern of subgroup J avian leukosis virus (ALV-J), SPF chickens were inoculated with ALV-J CHN06 via allantoic cavity on the eleventh day of embryonation ( 11 ED) and orally, ocu- lamasally, intramuscularly, subcutaneously, intra-abdominally on day 1 and intra-abdominally on day 7. At 1,2,4,6 w post inoculation, chickens were examined for ALV J viremia, ALV-J antibody and virus shedding. The results showed that the infection pattern of 11 ED group was predonminately V+ S+, while the oral and ocularnasal group were mainly V-S-.The infection patterns of the groups inoculated subcutaneously, intramuscularly and intra-abdominally on day 1 were mainly V+S+ .Transient viremla could be detected in the group of intra-abdommal inoculation on day 7.There was significant difference of seropositive rate at week 6 between the groups challenged on day 1 and day 7.The results showed that early prevention will be helpful in the eradication of ALV-J.
出处
《中国兽医杂志》
CAS
北大核心
2015年第6期14-16,共3页
Chinese Journal of Veterinary Medicine
基金
国家肉鸡产业技术体系(CARS-42-G09)
广东省科技计划项目(2012A020100001)
农业部公益性行业科研专项(2012-03055)
关键词
J亚群禽白血病病毒
接种途径
接种日龄
感染模式
subgroup J avian leukosis virus (ALV-J)
infection route
age
infection pattern