人参皂苷Rg1对小鼠创伤后应激障碍预防作用

PREVENTION OF GINSENOSIDE RG1 ON POST-TRAUMATIC STRESS DISORDER IN MICE

目的研究人参皂苷Rg1对小鼠创伤后应激障碍(PTSD)的预防作用。方法采用单次延长应激(SPS)方法建立PTSD小鼠模型,造模后生理盐水组、Rg1处理组、阳性对照组小鼠分别给予生理盐水、Rg1和舍曲林腹腔注射,连续8d;空白对照组小鼠接受生理盐水注射,不接受SPS刺激。造模后第8、10天,分别进行高架十字迷宫实验和强迫游泳实验。结果高架十字迷宫实验结果显示,Rg1处理组和生理盐水组模型小鼠进入开放臂的次数百分比均显著低于空白对照组(F=11.06,t=4.505、4.564,P<0.01),Rg1处理组和生理盐水组比较差异无显著性(P>0.05);而阳性对照组模型小鼠进入开放臂的次数百分比明显高于Rg1处理组(t=3.440,P<0.05),并接近空白对照组小鼠。在强迫游泳实验中,Rg1处理组和生理盐水组模型小鼠在水中的不动时间显著长于空白对照组和阳性对照组小鼠(F=8.291,P<0.01)。结论与临床常用5-羟色胺再摄取抑制剂类抗抑郁药舍曲林相比,人参皂苷Rg1对PTSD小鼠的焦虑和抑郁情绪反应无明显预防作用。

Objective To study the prevention of ginsenoside Rgl on mice with post-traumatic stress disorder （PTSD）. Methods Mouse models of PTSD were created using single prolonged stress （SPS）. The models were then randomized to normal saline group, Rgl treated group and sertraline group, they were given intraperitoneal injection of normal saline, Rgl and sertra- line, respectively, for 8 days; for those in the blank control group were given only normal saline injection without SPS. On days 8 and 10 after the models were created, elevated plus-maze test （EPMT） and forced swimming test （FST） were performed. Resuits EPMT showed that the percentage of the mice entering the open arms in Rglgroup and normal saline group was lower than that in the blank control group, the difference being significant （F = 11.06 ; t = 4. 505,4. 564 ; P 〈 0.01 ）, and that between Rgl group and normal saline group was not （P〉0.05）. The percentage of times entering the open arms in mice of the sertraline group was much higher than that in the Rg1-treated group （t =3.440 ,P〈0.05）, approaching the mice in the blank-control. In FST, the immobility time in water in mice of Rg1-treated group and normal saline group was much longer than that in mice of blank-control and sertraline-treated groups （F=8.291,P〈0.01）. Conclusion Comparing with sertraline, a clinical commonly used antide- pressive drug, Rgl has no antidepressive effect on mice with post-traumatic stress disorder.