DHA诱导小鼠乳癌4T1细胞凋亡及其对死亡受体蛋白表达的影响

APOPTOSIS OF MOUSE 4T1 BREAST CANCER CELL INDUCED BY DHA AND ITS DFFECTS ON EXPRESSIONS OF DEATH RECEPTORS

目的 研究二十二碳六烯酸（DHA）对小鼠乳癌4T1细胞凋亡的诱导作用及其对死亡受体（DR）蛋白表达的影响。方法 体外培养4T1细胞,应用MTT方法观察不同浓度的DHA对4T1细胞增殖活力的影响;Annexin V/PI双染流式细胞术分析不同浓度DHA对4T1细胞凋亡率影响;采用Western blotting方法检测DR4、DR5和肿瘤坏死因子相关凋亡诱导配体（TRAIL）表达的变化。结果 采用不同浓度DHA处理后,4T1细胞增殖活力较对照组显著下降（F=84.62,q=16.54~25.31,P〈0.05）;细胞凋亡率明显增高（F=287.68,q=32.74~56.58,P〈0.05）;DR5和TRAIL表达均明显上调（F=64.75、149.72,q=9.32~51.59,P〈0.05）。结论 DHA可能通过DR途径抑制小鼠4T1乳癌细胞的生长,诱导其凋亡。

Objective To investigate the effects of docosahexaenoic acid （DHA） on apoptosis of mouse 4T1 breast cancer cells and its influence on expressions of death receptor protein. Methods 4T1 cells were cultured in vitro. The effect of different concentrations of DHA on vitality of cell multiplication was observed by MTT assay. The cell apoptosis was analyzed by flow cytometry with Annexin/PI staining. The expressions of TRAIL and death receptor proteins were analyzed by Western blotting. Results After treatment with different-concentration DHA, the viability of 4T1 ceils was much lower than that in the control （F=84.62,q=16.S4-25.31,P〈0.05）, apoptosis increased （F=287.68,q=32.74-56.58,P〈0.05）, and the expressions of TRAIL and DR5 elevated （F=64.75,149.72;q=9.32--51.59;P〈0.05）. Conclusion DHA is likely to inhibit the growth and induce death of 4TI breast cancer cells in mouse through the path of death receptor.