摘要
目的:建立同时测定腰痛片(杜仲叶、赤芍、补骨脂、续断、狗脊等)中活性成分芍药苷、川续断皂苷Ⅵ、补骨脂素与异补骨脂素含量的方法。方法:采用HPLC法,色谱柱为Inert Sustain C18柱(250 mm×4.6 mm,5μm),流动相为乙腈(A)-水(B),梯度洗脱(0 min,10%A;60 min,50%A),流速1 m L·min-1,柱温25℃,检测波长为212 nm(川续断皂苷Ⅵ)、238 nm(芍药苷、补骨脂素和异补骨脂素),进样量10μL。结果:芍药苷、川续断皂苷Ⅵ、补骨脂素与异补骨脂素质量浓度分别在201.6-4 032μg·m L-1(r=0.999 5)、80-2 000μg·m L-1(r=0.999 9)、11.04-220.8μg·m L-1(r=1.00 0)、8.7-174μg·m L-1(r=0.999 7)范围内与峰面积线性关系良好,平均回收率(n=9)分别为100.6%、100.6%、100.4%、99.93%,精密度试验RSD均小于0.80%。结论:经方法学验证,该法能同时测定4种成分的含量,适用于腰痛片的质量控制。
Objective:To determine the content of four major components(paeoniflorin,asperosaponin Ⅵ,psoralen and isopsoralen) in Yaotong tablets by high performance liquid chromatography.Methods:The determination was conducted on the Insert Sustain-C18column(250 mm × 4.6 mm,5 μm) with the mobile phase of acetonitrile(A)-water(B),gradient elution(0 min,10% A;60 min,50% A) at the flow rate of 1 m L·min-1;the column temperature was at 25 ℃;the detection wavelength was 212 nm for asperosaponin Ⅵ,and 238 nm for paeoniflorin,psoralen and isopsoralen;and the injection volume was 10 μL.Results:Good linear relationship of paeoniflorin,asperosaponin Ⅵ,psoralen and isopsoralen between peak area and concentrations were noted within 201.6-4 032 μg·m L-1(r = 0.999 5),80-2 000 μg·m L-1(r = 0.999 9),11.04-220.8 μg·m L-1(r = 1.000),and 8.7-174 μg·m L-1(r = 0.999 7),respectively.The average recoveries(n = 9) were 100.6%,100.6%,100.4%,99.93%,RSD of precision test was less than 0.8%.Conclusion:Methodology verification proved that the method could be applied to simultaneously determine four components and control the quality Yaotong tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2015年第6期1099-1104,共6页
Chinese Journal of Pharmaceutical Analysis