促红细胞生成素对缺氧缺血性脑损伤新生大鼠脑组织Fas/FasL表达的影响

Effect of erythropoietin on Fas/FasL expression in brain tissues of neonatal rats with hypoxic ischemic brain damage

目的探讨促红细胞生成素(EPO)对新生大鼠缺血缺氧性脑损伤(HIBD)后脑组织中凋亡相关因子及其配体(Fas/Fas L)表达的影响。方法选120只7日龄新生大鼠,随机分为3组,假手术组、缺血缺氧组(模型组)和红细胞生成素组(实验组),设5个时间点:HIBD模型建立后6,12,24,48和72 h。每组于各个时间点分别处死8只大鼠,取脑组织,进行HE染色,观察脑细胞形态学改变,免疫组化染色观察Fas/Fas L的表达。结果模型组,Fas和Fas L的表达于HIBD模型建立后48 h达峰值,至72 h明显下降;与假手术组相比,模型组在各个时间点的Fas和Fas L表达均明显增加(P<0.05)。与模型组相比,实验组的Fas和Fas L的表达在各个时间点均有显著降低(P<0.05)。结论 EPO可通过减少HIBD新生大鼠脑组织中Fas及Fas L的表达,抑制脑组织缺氧缺血损伤后的细胞凋亡。

Objective To investigate the effect of erythropoietin （ EPO） on factor -related apoptosis and its ligand （ Fas/FasL ） expression in brain tissues of neonatal rats with hypoxic ischemic brain damage （ HIBD ） .Methods One hundred and twenty newborn rats （ 7 days years old ） were selected and randomly divided into 3 groups： sham group, hypoxic ischemic brain damage group （ model group ） and EPO treatment group （ test group ） , each group was further divided into five time points：6, 12, 24, 48 and 72 h after HIBD model was established. Eight rats in each group were killed at different time points.After which samples of brain tissues were harvested.Change of brain cell morphology was observed by HE staining and expression of Fas/FasL was observed by immunohistochemical staining.Results In model group, expressions of Fas and FasL reached the peak at 48 h after HIBD model was established and decreased at 72 h.Compared with sham group, the expressions of Fas and FasL in model group at each time point were much higher, and the difference was statistically significant （ P 〈0.05 ） .In test group, expressions of Fas and FasL at each time point were significantly lower than in model group （ P 〈0.05 ） .Conclusion Cell apoptosis in the brain tissue after hypoxic -ischemic damage could be remarkably inhi-bited by EPO through reduction of Fas and FasL expression in brain tissue of neonatal rats with HIBD.