变应性鼻炎患者口咽部菌群分析

Analysis of oropharynxmicrobiota in allergic rhinitis patients

目的研究正常人与过敏性鼻炎患者口咽部菌群差异,探讨菌群定植的变化对过敏性鼻炎发生存在的影响。方法利用PCR-DGGE方法分析正常人与过敏性鼻炎患者口咽部菌群定植,并利用切胶测序分析DGGE图谱中两组样本之间的差异菌群。结果正常人与过敏性鼻炎患者口咽部菌群定植存在显著差异,与正常组相比,过敏性鼻炎患者口咽部菌群多样性显著增大,且类杆菌门的Prevotella pallens,厚壁菌门的Veillonella atypica、Veillonella parvula、Streptococcus salivarius,变形菌门的假单胞菌属Pseudomonas fluorescens的含量均高于正常人。结论过敏性鼻炎患者口咽部菌群多样性显著高于正常人,且以厚壁菌门和变形菌门的增加为特征。

Objective To analysis the difference of microbiota colonization in oropharynx between normal and allergic rhinitis patients,discuss the influence of airway microbiota colonization changes on the development of allergic rhinitis. Methods The PCR-DGGE was utilized to analysis the bacteria colonization in oropharynx between normal and allergic rhinitis patients,then gel cutting and sequencing was used to check the different species between this two groups. Results The airway microbiota colonization in normal and allergic rhinitis patients showed a significantly difference,compared with control group,the diversity of airway microbiota significantly increased in oropharynx of allergic rhinitis patients. While the Prevotella pallens in Bacteroids,Veillonella atypica,Veillonella parvula,Streptococcus salivarius in Firmicutes, Pseudomonas fluorescens in Proteobacteria were higher than control group. Conclusion The diversity of airway microbiota in oropharynx of allergic rhinitis patients is significantly higher than that in control group,and was specified with the increase of Firmicutes and Proteobacteria.