不同培养基对SELDI-TO FMS细菌蛋白指纹图谱的影响

Effects of different media on SELDI-TOF MS fingerprints of bacterial proteins

目的检测不同培养基对细菌蛋白SELDI-TOF MS指纹图谱的影响。方法 3株参考菌株分别接种在不同培养基中,利用SELDI-TOF MS检测蛋白指纹图谱,分析其异同。结果在所有培养基中参考菌株ATCC 12600、ATCC 25922和ATCC 27853蛋白质谱图恒定表达的蛋白峰个数为11、11、9个,同时菌株在不同培养基中也表达少量其独特的蛋白峰。结论比较不同培养基上生长的同株菌蛋白指纹图谱,显示在不同培养基其细菌特征蛋白恒定表达。

Objective To investigate the effects of culture media on SELDI-TOF MS fingerprints of bacterial proteins. Methods The protein fingerprints of three reference strains derived from different culture media were analyzed to explore the effects of the culture media on the spectra. Results The number of protein peaks of the Reference strains ATCC 12600,ATCC 25922 and ATCC 27853 stably expressed on protein spectra was 11,11 and 9 respectively in all culture media; the strains also expressed a small amount of their unique protein peaks. Conclusion Comparison of the protein fingerprints of the same strain cultured with different media shows that its characteristic protein peaks express stably in spite of different culture media.