尿液CMV-DNA联合血清CMV-IgM检测对婴幼儿巨细胞病毒感染的诊断价值

Value of urine cytomegalovirus-DNA detection combined with serum cytomegalovirus-IgM detection in diagnosis of cytomegalovirus infection

目的：研究尿液巨细胞病毒DNA（CMV-DNA）联合血清巨细胞病毒特异抗体（CMV-IgM）检测对婴儿巨细胞病毒（CMV）感染的诊断价值。方法：选取2012年8月~2014年8月聊城市人民医院疑似CMV感染的婴幼儿70例,应用化学发光法检测血清CMV-IgM,采用实时荧光定量PCR法（FQ-PCR）检测尿液CMV-DNA,比较抗病毒治疗前后的检测结果。结果：单独检测尿CMV-DNA的阳性率为62.86%（44/70）,单独检测血清CMV-IgM的阳性率为55.71%（39/70）,两者比较差异无统计学意义（P〉0.05）;两者联合检测的阳性率为77.14%（54/70）,与两种方法单独检测比较差异均有统计学意义（P〈0.05）;抗病毒治疗后两种检测结果均显著降低,与治疗前比较差异有统计学意义（P〈0.05）。结论：尿液CMVDNA联合血清CMV-IgM检测能显著提高婴儿CMV感染的阳性率。

Objective： To research the value of urine cytomegalovirus- DNA（ CMV- DNA） detection combined with serum cytomegalovirus- IgM（ CMV- IgM） detection in diagnosis of cytomegalovirus（ CMV） infection. Methods： Seventy infants suspected of CMV infection were selected from the hospital from August 2012 to August 2014,chemiluminescence method was used to detect serum CMV-IgM,real- time fluorescent quantitative- PCR（ FQ- PCR） was used to detect urine CMV- DNA,the detection results before and after antiviral treatment were compared. Results： The positive rate of urine CMV- DNA detection was 62. 86%（ 44 /70）,the positive rate of serum CMV- IgM detection was 55. 71%（ 39 /70）,there was no statistically significant difference（ P〈0. 05）; the positive rate of joint detection was 77. 14%（ 54 /70）,compared with single detection,there were statistically significant differences（ P〈0. 05）; after antiviral treatment,the detection results of the two methods decreased significantly,compared before treatment,there were statistically significant differences（ P〈0. 05）. Conclusion： Urine CMV- DNA detection combined with serum CMV- IgM detection can significantly improve the positive detection rate of cytomegalovirus infection among infants.