IGFBP-rP1通过调控MEK/ERK信号通路对抑制子宫内膜癌HEC-1A细胞增殖的影响及机制探讨

Inhibiting effect of IGFBP-rP1 on proliferation of endometrial cancer HEC-1A cells by regulating MEK / ERK signal pathway and the mechanism

目的:探讨外源性胰岛素样生长因子结合蛋白相关蛋白1(IGFBP-rP1)联合MEK/ERK信号通路抑制剂PD98059对子宫内膜癌细胞HEC-1A增殖的影响及其机制。方法:体外培养人子宫内膜癌细胞HEC-1A细胞,向培养基中添加不同浓度人重组胰岛素生长因子结合蛋白相关蛋白1(rh IGFBP-rP1)和PD98059,上调IGFBP-rP1的水平,采用Cell Counting Kit-8(CCK-8)法检测不同浓度rh IGFBP-P1及rh IGFBP-P1联合MEK/ERK信号通路抑制剂PD98059对HEC-1A细胞增殖的影响,并用蛋白免疫印迹(western blot)法分析不同浓度rh IGFBP-rP1作用下子宫内膜癌细胞HEC-1A细胞总的ERK1/2和磷酸化ERK1/2水平变化。结果:添加rh IGFBP-rP1子宫内膜癌细胞HEC-1A增殖受到明显抑制,其抑制作用呈浓度和时间依赖性(P<0.05);4μg/ml r IGFBP-rP1对HEC-1A增殖抑制作用随着时间延长越来越明显,12、24、48、72 h增殖抑制率比较差异有统计学意义(P<0.05)。而MEK/ERK通路抑制剂PD98059能增强其抑制作用,r IGFBP-rP1(4μg/ml)与PD98059(25μmol/L)联用,以上各时间点细胞增殖抑制率分别增至(10.04±2.71)%、(17.02±1.58)%、(28.59±2.04)%、(35.29±1.12)%,各组比较,差异有统计学意义(P<0.05);Western blot示添加rh IGFBP-rP1,磷酸化的ERK1/2水平显著降低,磷酸化的ERK1/2水平与rh IGFBP-rP1呈剂量依赖性。结论:IGFBP-rP1可以通过MEK/ERK信号通路抑制细胞增殖。

Objective： To explore the inhibiting effect of exogenous insulin like growth factor binding protein- related protein 1（ IGFBP- rP1） on proliferation of endometrial cancer HEC- 1A cells by MEK/ERK signal pathway suppressor PD98059 and the mechanism. Methods： Human endometrial cancer HEC- 1A cells were cultured in vitro,different doses of rh IGFBP- rP1 and PD98059 were added into the culture medium to upregulate IGFBP- rP1 level,cell counting kit- 8（ CCK- 8） method was used to detect the effects of different doses of rh IGFBP- P1 and rh IGFBP- P1 combined with MEK / ER signal pathway suppressor PD98059 on proliferation of endometrial cancer HEC- 1A cells; western blot was used to analyze the changes of total ERK1 /2 and p ERK1 /2 in endometrial cancer HEC- 1A cells after treated with different doses of rh IGFBP- rP1. Results： After adding rh IGFBP- rP1,the proliferation of endometrial cancer HEC- 1A cells was inhibited significantly,showing a concentration- dependent and time- depedent manner（ P〈0. 05）; the inhibiting effect of 4 μg/ ml r IGFBP- rP1 on proliferation of endometrial cancer HEC- 1A cells enhanced with time,there was statistically significant difference in the inhibiting rate among 12,24,48,and 72 hours（ P〈0. 05）. MEK / ERK signal pathway suppressor PD98059 enhanced the inhibiting effect,when combining with r IGFBP- rP1（ 4 μg / ml） and PD98059（ 25 μmol / L）,the inhibiting rates at 12,24,48,and 72 hours increased to（ 10. 04 ± 2. 71） %,（ 17. 02 ± 1. 58） %,（ 28. 59 ± 2. 04） %,and（ 35. 29 ± 1. 12） %,respectively,there was statistically significant difference（ P〈0. 05）; western blot showed that after adding rh IGFBP- rP1,p ERK1 /2 level decreased significantly,showing a dose- dependent manner. Conclusion： IGFBP- rP1 can inhibit proliferation of endometrial cancer HEC- 1A cells by regulating MEK / ERK signal pathway.