荧光假单胞菌生物膜的定性观察及影响因素研究

The qualitative observation of biofilm formation for Pseudomonas fluourcens and its affective factors

通过结晶紫染色方法对荧光假单胞菌生物膜的形成进行定性观察表明，该菌在培养12h后开始团聚硅胶片上形成生物膜，48h形成较为致密的生物膜结构。分别研究NaCI浓度、温度、pH对荧光假单胞菌生物膜生长影响，在NaCl浓度0．05-0．1mol／L，培养温度25℃，pH为7．5，荧光假单胞菌较快形成生物膜；当碳源为葡萄糖和氮源为大豆蛋白时，荧光假单胞菌较容易形成生物膜。

In the experiment, the qualitative observation was carried out for the formation of Pseudomonas fluourcens biofilms by using crystal voilet staining. The results showed that the bacteria could aggregate on the silicone piece and formed the initial biofilm after 12 hours culture, After 48 hours, the biofilm become relatively dense. The effects of NaCI concentration, temperature, pH, appropriate carbon and nitrogen source on the growth of biofilm were evaluated for Pseudomonas fluourcens. When NaCI concentration was 0.05-0.1 tool/L, culture temperature was 25 ℃, and pH value was 7.5, Pseudomonas fluourcens could form biofilms more easily. The appropriate carbon and nitrogen source were respectively glucose and soybean protein for Pseudomonas fluourcens to form biofilms as soon as possible.